DOK7 (Downstream of kinase 7) is a 55 kDa member of the DOK family of cytoplasmic adaptor proteins. It links the acetylcholine receptor and the receptor tyrosine kinase MuSK in skeletal and cardiac muscle. Mutations can cause familial myasthenic syndromes. DOK7 contains pleckstrin homology, phosphotyrosine-binding and SH2 domains, and a C-terminal nuclear export signal. Multiple isoforms exist.
DOK7 Antibody (OTI2C12) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-72491
Discontinued Product
NBP2-72491 has been discontinued.
View all DOK7 products.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI2C12
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length human recombinant protein of human DOK7(NP_775931) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
52.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DOK7 Antibody (OTI2C12) - Azide and BSA Free
Western Blot: DOK7 Antibody (OTI2C12)Azide and BSA Free [NBP2-72491]
Western Blot: DOK7 Antibody (OTI2C12) - Azide and BSA Free [NBP2-72491] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK7.Applications for DOK7 Antibody (OTI2C12) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: DOK7
Long Name
Downstream of Tyrosine Kinase 7
Alternate Names
C4orf25, CMS1B
Gene Symbol
DOK7
Additional DOK7 Products
Product Documents for DOK7 Antibody (OTI2C12) - Azide and BSA Free
Certificate of Analysis
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Product Specific Notices for DOK7 Antibody (OTI2C12) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
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- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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