Dystrophin Antibody (3A11) - Azide and BSA Free

Novus Biologicals | Catalog # H00001756-M07

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3A11

Format

Azide and BSA Free
View all Dystrophin Primary Antibodies »

Product Specifications

Immunogen

DMD (AAH28720.1, 1 a.a. ~ 635 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSDSMGEEDLLSPPQDTSTGLEEVMEQLNNSFPSSRGHNVGSLFHMADDLGRAMESLVSVMTDEEGAE

Specificity

DMD - dystrophin (muscular dystrophy, Duchenne and Becker types) (3A11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Dystrophin Antibody (3A11) - Azide and BSA Free

Western Blot: Dystrophin Antibody (3A11) [H00001756-M07]

Western Blot: Dystrophin Antibody (3A11) [H00001756-M07]

Western Blot: Dystrophin Antibody (3A11) [H00001756-M07] - Analysis of DMD expression in transfected 293T cell line by DMD monoclonal antibody (M07), clone 3A11. Lane 1: DMD transfected lysatE (72.2 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation: Dystrophin Antibody (3A11) [H00001756-M07]

Immunoprecipitation: Dystrophin Antibody (3A11) [H00001756-M07]

Immunoprecipitation: Dystrophin Antibody (3A11) [H00001756-M07] - Analysis of DMD transfected lysate using anti-DMD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DMD rabbit polyclonal antibody.
ELISA: Dystrophin Antibody (3A11) [H00001756-M07]

ELISA: Dystrophin Antibody (3A11) [H00001756-M07]

ELISA: Dystrophin Antibody (3A11) [H00001756-M07] - Detection limit for recombinant GST tagged DMD is 0.3 ng/ml as a capture antibody.

Applications for Dystrophin Antibody (3A11) - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Dystrophin

The dystrophin gene is the largest gene found in nature, measuring 2.4 Mb. The gene was identified through a positional cloning approach, targeted at the isolation of the gene responsible for Duchenne (DMD) and Becker (BMD) Muscular Dystrophies. DMD is a recessive, fatal, X-linked disorder occurring at a frequency of about 1 in 3,500 new-born males. BMD is a milder allelic form. In general, DMD patients carry mutations which cause premature translation termination (nonsense or frame shift mutations), while in BMD patients dystrophin is reduced either in molecular weight (derived from in-frame deletions) or in expression level. The dystrophin gene is highly complex, containing at least eight independent, tissue-specific promoters and two polyA-addition sites. Furthermore, dystrophin RNA is differentially spliced, producing a range of different transcripts, encoding a large set of protein isoforms. Dystrophin (as encoded by the Dp427 transcripts) is a large, rod-like cytoskeletal protein which is found at the inner surface of muscle fibers. Dystrophin is part of the dystrophin-glycoprotein complex (DGC), which bridges the inner cytoskeleton (F-actin) and the extra-cellular matrix. [provided by RefSeq]

Alternate Names

BMDDXS272, CMD3B, DXS142, DXS164, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, dystrophin, dystrophin (muscular dystrophy, Duchenne and Becker types), includes DXS142

Entrez Gene IDs

1756 (Human)

Gene Symbol

DMD

UniProt

AAH28720 (Human)

Additional Dystrophin Products

Product Documents for Dystrophin Antibody (3A11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Dystrophin Antibody (3A11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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