Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # PCRP-E2F6-1F8

Format

Azide and BSA Free
View all E2F6 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human E2F6 protein (Uniprot: O75461)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07268).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for E2F6 Antibody (PCRP-E2F6-1F8) - Azide and BSA Free

Flow Cytometry: E2F6 Antibody (PCRP-E2F6-1F8) - Azide and BSA Free [NBP3-08952]

Flow Cytometry: E2F6 Antibody (PCRP-E2F6-1F8) - Azide and BSA Free [NBP3-08952]

Flow Cytometry: E2F6 Antibody (PCRP-E2F6-1F8) - Azide and BSA Free [NBP3-08952] - Flow Cytometric Analysis of PFA-fixed HeLa cells. E2F6 Mouse Monoclonal Antibody (PCRP-E2F6-1F8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Immunocytochemistry/ Immunofluorescence: E2F6 Antibody (PCRP-E2F6-1F8) - Azide and BSA Free [NBP3-08952]

Immunocytochemistry/ Immunofluorescence: E2F6 Antibody (PCRP-E2F6-1F8) - Azide and BSA Free [NBP3-08952]

Immunocytochemistry/Immunofluorescence: E2F6 Antibody (PCRP-E2F6-1F8) - Azide and BSA Free [NBP3-08952] - Immunofluorescence Analysis of HeLa cells using E2F6 Mouse Monoclonal Antibody (PCRP-E2F6-1F8) followed by goat anti-mouse IgG-CF488 (green). Phalloidin (red).
Protein Array: E2F6 Antibody (PCRP-E2F6-1F8) - Azide and BSA Free [NBP3-08952]

Protein Array: E2F6 Antibody (PCRP-E2F6-1F8) - Azide and BSA Free [NBP3-08952]

Protein Array: E2F6 Antibody (PCRP-E2F6-1F8) - Azide and BSA Free [NBP3-08952] - Analysis of Protein Array containing more than 19,000 full-length human proteins using E2F6 Mouse Monoclonal Antibody (PCRP-E2F6-1F8).

Applications for E2F6 Antibody (PCRP-E2F6-1F8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: E2F6

E2F6 encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms.

Alternate Names

E2F transcription factor 6, E2F-6MGC111545, transcription factor E2F6

Gene Symbol

E2F6

Additional E2F6 Products

Product Documents for E2F6 Antibody (PCRP-E2F6-1F8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for E2F6 Antibody (PCRP-E2F6-1F8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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