E2F7 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-57294

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: MEVNCLTLKDLISPRQPRLDFAVEDGENAQKENIFVDRSRMAPKTPIKNEPIDLSKQKKFTPERNPITPVKLVDRQQAEPW

Reactivity Notes

Mouse 87%, Rat 88%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for E2F7 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: E2F7 Antibody [NBP2-57294]

Immunocytochemistry/ Immunofluorescence: E2F7 Antibody [NBP2-57294]

Immunocytochemistry/Immunofluorescence: E2F7 Antibody [NBP2-57294] - Staining of human cell line U-251 MG shows localization to nuclear speckles. Antibody staining is shown in green.
E2F7 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: E2F7 Antibody - BSA Free [NBP2-57294]

Chromatin Immunoprecipitation-exo-Seq: E2F7 Antibody - BSA Free [NBP2-57294]

ChIP-Exo-Seq composite graph for Anti-E2F7 (NBP2-57294) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for E2F7 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1-10µg per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: E2F7

E2F transcription factors, such as E2F7, play an essential role in the regulation of cell cycle progression (Di Stefano et al., 2003 [PubMed 14633988]).

Alternate Names

E2F transcription factor 7, E2F-7, FLJ12981, transcription factor E2F7

Gene Symbol

E2F7

Additional E2F7 Products

Product Documents for E2F7 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for E2F7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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