eIF3e Antibody

Novus Biologicals | Catalog # NBP1-52116

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all eIF3e Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KLNQNSRSEAPN corresponding to C-Terminus according to NP_001559.1.

Epitope

C-KLNQNSRSEAPN

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for eIF3e Antibody

Western Blot: eIF3e Antibody [NBP1-52116]

Western Blot: eIF3e Antibody [NBP1-52116]

Western Blot: eIF3e Antibody [NBP1-52116] - Analysis of eIF3e in Daudi lysate (35ug protein in RIPA buffer) using NBP1-52116 at 0.03 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for eIF3e Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000

Western Blot

0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in Daudi lysates (calculated MW of 52.2 kDa band according to NP_001559.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eIF3e

Int6 is a candidate tumor suppressor in multiple neoplasms, and in particular, breast and lung cancers. The Int6 locus was initially identified as a common insertion site (CIS) in a genetic screen for transforming sequences in a breast cancer mouse model system. Insertion of mouse mammary tumor virus (MMTV) into this locus results in the production of an amino-terminal truncated gene product. Expression of the truncated Int6 product corresponds to cellular transformation in both in vivo and in vitro systems. This gene product plays a role in regulating translation initiation and is a component of the eIF3 translation initiation complex. There is evidence that suggests that Int6 may impart a negative role in the general translational machinery while promoting an increase in the expression of a subset of stressresponsive genes. Taken together, it is of great interest to further study the mechanism by which Int6 is involved in regulating cell growth.

Alternate Names

eIF-3 p48, eIF3e, eIF3-p48, EIF3S6EIF3-P48, Eukaryotic translation initiation factor 3 subunit 6, eukaryotic translation initiation factor 3 subunit E, eukaryotic translation initiation factor 3, subunit 6 (48kD), eukaryotic translation initiation factor 3, subunit 6 48kDa, eukaryotic translation initiation factor 3, subunit E, INT6eIF3-p46, mammary tumor-associated protein INT6, murine mammary tumor integration site 6 (oncogene homolog), Viral integration site protein INT-6 homolog

Entrez Gene IDs

3646 (Human)

Gene Symbol

EIF3E

UniProt

NP_001559.1 (Human)

Additional eIF3e Products

Product Documents for eIF3e Antibody

Certificate of Analysis

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Product Specific Notices for eIF3e Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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