Endothelial Lipase Antibody

Novus Biologicals | Catalog # NBP1-05031

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence C-RFNLRTSKDPEHEG corresponding to internal region according to NP_006024.1.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Endothelial Lipase Antibody

Western Blot: Endothelial Lipase Antibody [NBP1-05031]

Western Blot: Endothelial Lipase Antibody [NBP1-05031]

Western Blot: Endothelial Lipase Antibody [NBP1-05031] - Analysis of Endothelial Lipase in Human Liver lysate using NBP1-05031 at 0.3ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Endothelial Lipase Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in human liver lysates is reported in scientific literature (PMID: 17356047). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Endothelial Lipase

The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq]

Alternate Names

EC 3.1.1, EC 3.1.1.3, EDLendothelial lipase, ELlipoprotein lipase H, Endothelial cell-derived lipase, lipase, endothelial, PRO719

Entrez Gene IDs

9388 (Human)

Gene Symbol

LIPG

Additional Endothelial Lipase Products

Product Documents for Endothelial Lipase Antibody

Certificate of Analysis

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Product Specific Notices for Endothelial Lipase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Endothelial Lipase Antibody

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