EWSR1 Antibody - BSA Free

Novus Biologicals | Catalog # NB200-183

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EWSR1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide that maps to a region between residues 600 and the C-terminus (residue 656) of human Ewing sarcoma breakpoint region 1 using the numbering given in SwissProt entry Q01844 (GeneID 2130).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EWSR1 Antibody - BSA Free

Western Blot: EWSR1 Antibody [NB200-183]

Western Blot: EWSR1 Antibody [NB200-183]

Western Blot: EWSR1 Antibody [NB200-183] - Detection of Human and Mouse EWS by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EWS antibody NB200-183 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunoprecipitation: EWSR1 Antibody [NB200-183]

Immunoprecipitation: EWSR1 Antibody [NB200-183]

Immunoprecipitation: EWSR1 Antibody [NB200-183] - Detection of human EWSR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EWSR1 antibody NB200-183 used for IP at 6 ug per reaction. EWSR1 was also immunoprecipitated by rabbit anti-EWSR1 antibody NB200-182. For blotting immunoprecipitated EWSR1, NB200-183 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for EWSR1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:10000 - 1:25000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EWSR1

This gene encodes a putative RNA binding protein of unknown function. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products.

Long Name

RNA-binding protein EWS

Alternate Names

EWS, EWS oncogene

Entrez Gene IDs

2130 (Human)

Gene Symbol

EWSR1

UniProt

Q01844 (Human)

Additional EWSR1 Products

Product Documents for EWSR1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EWSR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EWSR1 Antibody - BSA Free

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