Exonuclease 1 Antibody

Novus Biologicals | Catalog # NB100-93394

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Exonuclease 1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HRNYSPRPESGT corresponding to internal region according to NP_003677.3, NP_006018.3, NP_569082.1.

Specificity

This antibody is expected to recognise all three reported isoforms (NP_003677.3; NP_006018.3 and NP_569082.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Exonuclease 1 Antibody

Western Blot: Exonuclease 1 Antibody [NB100-93394]

Western Blot: Exonuclease 1 Antibody [NB100-93394]

Western Blot: Exonuclease 1 Antibody [NB100-93394] - (0.2ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Exonuclease 1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.2 - 0.5 ug/ml
Application Notes
WB: Approx. 90 kDa band observed in lysates in cell lines HEK293 and A549 (calculated MW of 89.1 kDa band according to NP_003677.3). An additional band of unknown identity was also consistently observed at 38 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Exonuclease 1

This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq]

Alternate Names

Exo1, EXOI, Exonuclease I

Entrez Gene IDs

9156 (Human)

Gene Symbol

EXO1

UniProt

Q9UQ84 (Human)

Additional Exonuclease 1 Products

Product Documents for Exonuclease 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Exonuclease 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Exonuclease 1 Antibody

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