Ferroportin/SLC40A1 Antibody (1308C) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80731

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1308C expressed in HEK293

Format

Azide and BSA Free
View all Ferroportin/SLC40A1 Primary Antibodies »

Product Specifications

Immunogen

This Ferroportin/SLC40A1 Antibody (1308C) - Azide and BSA Free was developed against a synthetic peptide made to an internal portion of human Ferroportin 1 (within residues 250-320). [UniProt# Q9NP59]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ferroportin/SLC40A1 Antibody (1308C) - Azide and BSA Free

Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody (1308C) - Azide and BSA Free [NBP2-80731]

Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody (1308C) - Azide and BSA Free [NBP2-80731]

Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody (1308C) - Azide and BSA Free [NBP2-80731] - Ferroportin/SLC40A1 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Ferroportin/SLC40A1 Antibody (1308C) (Catalog # NBP2-75923) at 1 ug/mL for 1 hour at room temperature followed by incubation with the Ant
Flow Cytometry: Ferroportin/SLC40A1 Antibody (1308C) - Azide and BSA Free [NBP2-80731]

Flow Cytometry: Ferroportin/SLC40A1 Antibody (1308C) - Azide and BSA Free [NBP2-80731]

Flow Cytometry: Ferroportin/SLC40A1 Antibody (1308C) - Azide and BSA Free [NBP2-80731] - HEK293 human embryonic kidney cell line transfected with human Ferroportin/SLC40A1 and eGFP was stained with either (A) Rabbit Anti-Human Ferroportin/SLC40A1 Antibody (1308C) (NBP2-75923) or (B) Rabbit IgG Isotype Control (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Image from the standard format of this antibody.

Applications for Ferroportin/SLC40A1 Antibody (1308C) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.25 ug/ml

Immunohistochemistry

1 ug/ml

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ferroportin/SLC40A1

Ferroportin is a 12-transmembrane domain protein, belonging to the major facilitator superfamily of transporters of small molecules, that is localized to the plasma membrane. Human Ferroportin has a theoretical molecular weight of 62.5 kDa. Ferroportin (FPN1 or SLC40A1) functions as an iron-regulated transporter (highly expressed in placenta, intestine, muscle, spleen, macrophages etc.) and is the receptor for the iron-regulatory hormone, hepcidin. In iron metabolism, FPN1 plays a key role in intestinal iron absorption as well as cellular iron release and mediates iron absorption in the presence of ferroxidases, hephaestin (HP) and/or ceruloplasmin (CP). FPN1 is implicated in iron export from duodenal epithelial cells and in the transfer of iron between maternal and fetal circulation. FPN1 transports iron in the ferrous form whereas plasma transferrin only binds iron's ferric form. Ferroxidases are key players in oxidizing iron transported by FPN1 and without the activity of ferroxidases, FPN1 is internalized followed by degradation. While other cell types utilize the circulating or GPI-linked multicopper ferroxidase CP for FPN1, intestinal cells utilize a membrane-bound HP, a paralog of CP that also show interaction with FPN1 (1).

FPN1 regulation is dependent on the cell type and involves transcriptional, posttranscriptional, and posttranslational mechanisms including hepcidin-mediated endocytosis and proteolysis. Hepcidin controls the concentration of FPN1 in the membrane, with hepcidin deficiency resulting in iron overload (high iron) and hepcidin excess leading to iron restriction and anemia (2). Ferroportin disease or hemochromatosis type 4 (HFE4) is associated with distinct FPN1 variants with either reduced FPN1 cell surface expression/iron export capacity or hepcidin resistance and iron overload (3, 4).

References

1. De Domenico I, Ward DM, Kaplan J. (2011) Hepcidin and ferroportin: the new players in iron metabolism. Semin Liver Dis. 31(3):272-9. PMID: 21901657

2. Drakesmith H, Nemeth E, Ganz T. (2015) Ironing out Ferroportin. Cell Metab. 22(5):777-87. PMID: 26437604

3. Pietrangelo A. (2017) Ferroportin disease: pathogenesis, diagnosis and treatment. Haematologica. 102(12):1972-1984. PMID: 29101207

4. Vlasveld LT, Janssen R, Bardou-Jacquet E, Venselaar H, Hamdi-Roze H, Drakesmith H, Swinkels DW. (2019) Twenty Years of Ferroportin Disease: A Review or An Update of Published Clinical, Biochemical, Molecular, and Functional Features. Pharmaceuticals (Basel). 12(3). pii: E132. PMID: 31505869

Long Name

Solute Carrier Family 40 Member 1

Alternate Names

FPN1, HFE4, IREG1, MST079, MTP1, SLC11A3, SLC40A1

Gene Symbol

SLC40A1

Additional Ferroportin/SLC40A1 Products

Product Documents for Ferroportin/SLC40A1 Antibody (1308C) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Ferroportin/SLC40A1 Antibody (1308C) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Ferroportin/SLC40A1 Antibody (1308C) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Ferroportin/SLC40A1 Antibody (1308C) - Azide and BSA Free and earn rewards!

Have you used Ferroportin/SLC40A1 Antibody (1308C) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies