Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Chimpanzee (100%), Orangutan (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Simple Western, Immunoprecipitation

Cited:

Western Blot, Immunoassay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 377 and 427 of human Flotillin 1 using the numbering given in entry NP_005794.1 (GeneID 10211).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Flotillin-1 Antibody - BSA Free (NBP1-79022) is a polyclonal antibody validated for use in WB, Simple Western and IP. Anti-Flotillin-1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Flotillin-1 Antibody - BSA Free

Western Blot: Flotillin-1 Antibody [NBP1-79022]

Western Blot: Flotillin-1 Antibody [NBP1-79022]

Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.
Simple Western: Flotillin-1 Antibody [NBP1-79022]

Simple Western: Flotillin-1 Antibody [NBP1-79022]

Simple Western: Flotillin-1 Antibody [NBP1-79022] - Simple Western lane view shows lysates of Exosome Standards (A549 cell line) (NBP3-11645), exosome standards (HT29 cell line) (NBP3-11685), and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for Flotillin-1 at approximately 51 kDa (as indicated) using 1:250 ug/mL of Flotillin-1 antibody (NBP1-79022). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Flotillin-1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg

Simple Western

1:250

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE

Reviewed Applications

Read 1 review rated 5 using NBP1-79022 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Flotillin-1

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

Alternate Names

flotillin 1, flotillin-1, integral membrane component of caveolae

Gene Symbol

FLOT1

UniProt

NP_005794.1 (Human)

Additional Flotillin-1 Products

Product Documents for Flotillin-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Flotillin-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Flotillin-1 Antibody - BSA Free

Customer Reviews for Flotillin-1 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
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1 Stars
0%

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  • Flotillin-1 Antibody
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Cortical neurons
    Species: Mouse
    Verified Customer | Posted 09/06/2017
    Flotillin-1 staning is detected in neurites and cell bodies in mouse cortical neuros (dilution: 1:100 [red], DAPI: blue).
    Flotillin-1 Antibody - BSA Free NBP1-79022

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