GluR2/3 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat, Chicken, Zebrafish
  • Specificity
    This antibody is specific for the 100 kDa GluR2/3 in Western blots of rat brain lysates.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    GluR2/3
  • Formulation
    100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of GluR2/3 by Western Blot. Western blot of rat hippocampal lysate showing the specific immunolabeling of the approximately 100 kDa GluR2/3 protein.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ ‑20° C is recommended. Product is stable at ≤‑20° C for at least 1 year.
Background: GluR2/3
Rat GluR2 is a 100 kDa, 862 amino acid, 4-transmembrane (TM) glycoprotein that belongs to the ionotrophic/glutamate-gated ion channel family. Due to an impenetrate TM region 2, the N-terminus is extracellular and C-terminus intracellular. GluR2 is the most widely expressed of four AMPA receptor subunits, and contributes to the formation of a functional, presumably heterotetrameric glutamate receptor. AMPA receptors mediate fast excitatory transmission on postsynaptic membranes. The GluR2 subunit in particular regulates Ca, Zn and Na entry into the cell. It is unique among AMPA subunits in that it contains an Arginine in the second transmembrane segment (amino acid 607). This accounts for its ability to regulate ion flow. Upon neuronal insult, the Arginine can be converted to Glutamine, which allows for rapid Ca++ influx and the initiation of apoptosis. Alternatively, GluR2 levels may drop, allowing for multiple GluR combinations that allow rapid Ca++ influx. GluR2 is regulated both by palmitoylation and phosphorylation. Palmitoylation at C610 results in decreased surface expression. Phosphorylation by PKC on S880, and by Src on Y876, induces internalization. Human and rat GluR2 are 99% aa identical. Rat GluR3 is 866 aa in length and shares 72% overall aa identity with GluR2, with 85% aa identity over the C-terminal 15 amino acids. It is apparently not phosphorylated. GluR3 has a Ca++-permissive Glutamine at amino acid 612, the homolog to GluR2 R607. GluR3 is restricted in its expression pattern and is found on cerebellar Purkinje and Golgi Type II cells, neurons of the lateral amygdala, and cells of the basal ganglia.
  • References:
    1. Keinanen, K. et al. (1990) Science 249:556.
    2. Dong, H. et al. (1997) Nature 386:279.
    3. Wyszynski, M. et al. (1999) J. Neurosci. 19:6528.
    4. Boulter, J. et al. (1990) Science 249:1033.
    5. Wang, J.Q. et al. (2005) Mol. Neurobiol. 32:327.
    6. Soundarapandian, M.M. et al. (2005) Mol. Neurobiol. 32:145.
  • Long Name:
    Glutamate Receptor 2/3
  • Alternate Names:
    AMPA 2; GluR2/3; glutamate receptor, ionotropic, AMPA 2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 42  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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