Glut3 Antibody (JA50-31)

Novus Biologicals | Catalog # NBP2-66872

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JA50-31 expressed in HEK293
View all Glut3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Glut3 aa 11-60 / 496. (SwissProt: P11169 Human; SwissProt: P32037 Mouse; SwissProt: Q07647 Rat)

Localization

Cell membrane. Perikaryon. Cell projection.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glut3 Antibody (JA50-31)

Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872]

Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872]

Glut3-Antibody-JA50-31-Western-Blot-NBP2-66872-img0007.jpg
Flow Cytometry: Glut3 Antibody (JA50-31) [NBP2-66872]

Flow Cytometry: Glut3 Antibody (JA50-31) [NBP2-66872]

Flow Cytometry: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of SH-SY5Y cells with Glucose Transporter GLUT3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Glut3 Antibody (JA50-31)

Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] -

Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of Glucose Transporter GLUT3 on different lysates with Rabbit anti-Glucose Transporter GLUT3 antibody at 1/5,000 dilution.Lane 1: HepG2 cell lysate (20 ug/Lane)Lane 2: RAW264.7 cell lysate (20 ug/Lane)Lane 3: C6 cell lysate (20 ug/Lane)Lane 4: Mouse brain tissue lysate (20 ug/Lane)Lane 5: Rat brain tissue lysate (20 ug/Lane)Predicted band size: 54 kDaObserved band size: 54 kDaExposure time: 24 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4c overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
Glut3 Antibody (JA50-31)

Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] -

Western Blot: Glut3 Antibody (JA50-31) [NBP2-66872] - Analysis of Glucose Transporter GLUT3 on different lysates with Rabbit anti-Glucose Transporter GLUT3 antibody at 1/1,000 dilution.Lane 1: HepG2-si NT cell lysate (10 ug/Lane)Lane 2: HepG2-si Glucose Transporter GLUT3 cell lysate (10 ug/Lane)Predicted band size: 54 kDaObserved band size: 54 kDaExposure time: 1 minute; ECL: merk4-20% SDS-PAGE gel.NBP2-66872 was shown to specifically react with Glucose Transporter GLUT3 in HepG2-si NT cells. Weakened band was observed when HepG2-si Glucose Transporter GLUT3 sample was tested. HepG2-si NT and HepG2-si Glucose Transporter GLUT3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for Glut3 Antibody (JA50-31)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glut3

Glucose is the major source of our energy and there are numerous isoforms of the glucose transporter in mammals, including Glut1, Glut2, Glut3, Glut4, Glut5, Glut6, Glut7, Glut8 and Glut9. The Glut5 gene located on the short arm of human chromosome 1 encodes a 501-amino acid facilitative glucose transporter. Glut5 mRNA is highly expressed in small intestine and to a lesser extent in kidney, skeletal muscle and adipose tissue. Glut5 plays a critical role in fructose absorption in the small intestine and its expression is highly induced when exposed to a fructose-enriched diet. Glut5 transporter expressed in human skeletal muscle is specifically localized to the plasma membrane, where it participates in regulating hexose transfer across the sarcolemma. Glut8, a novel glucose transporter-like protein, exhibits significant sequence similarity with the other members of sugar transporter family. Glut8 comprises 12 putative membrane-spanning helices and several conserved motifs, which are important for transport activity. In human tissues, Glut8 is predominantly expressed in testis and, to a lesser extent, in most other tissues including skeletal muscle, heart, small intestine and brain. In addition, the Glut8 glucose transport facilitator has a hormonally regulated testicular function.

Long Name

Glucose Transporter Type 3

Alternate Names

SLC2A3

Gene Symbol

SLC2A3

Additional Glut3 Products

Product Documents for Glut3 Antibody (JA50-31)

Certificate of Analysis

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Product Specific Notices for Glut3 Antibody (JA50-31)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glut3 Antibody (JA50-31)

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