GPIP137 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-22238
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Western Blot, Immunoprecipitation
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 550 and 600 of human Cell Cycle Associated Protein 1 using the numbering given in entry NP_005889.3 (GeneID 4076).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GPIP137 Antibody - BSA Free
Western Blot: GPIP137 Antibody [NBP2-22238]
Western Blot: GPIP137 Antibody [NBP2-22238] - Analysis of GPIP137 in human B-cell whole cell lysate using GPIP137 antibody. Image from verified customer review.Western Blot: GPIP137 Antibody [NBP2-22238]
Western Blot: GPIP137 Antibody [NBP2-22238] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22238 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.Immunoprecipitation: GPIP137 Antibody [NBP2-22238]
Immunoprecipitation: GPIP137 Antibody [NBP2-22238] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. NBP2-22238 used for IP at 6 ug/mg lysate. GPIP137 was also immunoprecipitated by rabbit anti-GPIP137 antibody NBP2-22237. For blotting immunoprecipitated GPIP137, NBP2-22238 was used at 1 ug/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.Immunoprecipitation: GPIP137 Antibody [NBP2-22238]
Immunoprecipitation: GPIP137 Antibody [NBP2-22238] - GPIP137 immunoprecipitation in human b-cells. Lane 1: 40 ug input, Lane 2: IgG, Lane 3: IP. Image from verified customer review.Applications for GPIP137 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg
Western Blot
1:2000 - 1:10000
Application Notes
IP and WB reported in a verified customer review.
Reviewed Applications
Read 4 reviews rated 4.3 using NBP2-22238 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: GPIP137
Alternate Names
activation/proliferation-associated protein 1, caprin 1, caprin-1, cell cycle associated protein 1, Cell cycle-associated protein 1, Cytoplasmic activation- and proliferation-associated protein 1, cytoplasmic activation/proliferation-associated protein-1, GPI-anchored membrane protein 1GPIP137, GPI-p137, M11S1GPI-anchored protein p137, Membrane component chromosome 11 surface marker 1, membrane component, chromosome 11, surface marker 1, p137GPI, RNA granule protein 105, RNG105GPIAP1
Gene Symbol
CAPRIN1
UniProt
Additional GPIP137 Products
Product Documents for GPIP137 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GPIP137 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for GPIP137 Antibody - BSA Free
Customer Reviews for GPIP137 Antibody - BSA Free (4)
4.3 out of 5
4 Customer Ratings
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Application: ImmunoprecipitationSample Tested: B-cell whole cell lysate 40ug and b-cellSpecies: HumanVerified Customer | Posted 12/04/2018GPIP137 immunoprecipitation in human b-cells. Lane 1: 40ug input, L2: IgG, L3: IP10ug antibody per mg lysate.
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Application: ImmunocytochemistrySample Tested: fibroblastsSpecies: HumanVerified Customer | Posted 11/18/2017GPIP137 in FibroblastsThe signal was strong, however; it showed up in the entire cell. Other CAPRIN1 IF images had no signal in the nucleus. So, it worked, but there are probably are antibodies validated for IF that should be compared side by side with this one to confirm. Diluted 1:250 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation.
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Application: ImmunoprecipitationSample Tested:Species: MouseVerified Customer | Posted 05/30/2016IP/Western of GPIP137 from mouse beta cell line
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Application: Western BlotSample Tested: B-cell whole cell lysate 40ugSpecies: HumanVerified Customer | Posted 12/22/2015GPIP137 Human B-cells
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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