GPRC5A is a member of the G protein-coupled receptor family, characterized by a signature 7- transmembrane domain motif. GPRC5A is a lung-specific tumor suppressor and may play a role in the interaction between retinoid acid and G protein signaling pathways. GPRC5A is also involved in embryonic development and epithelial cell differentiation.
GPRC5A/RAI3 Antibody (GPRC5A/7893) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-24315
![GPRC5A/RAI3 Antibody (GPRC5A/7893) - Azide and BSA Free Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) - Azide and BSA Free [NBP3-24315] -](https://resources.rndsystems.com/images/products/nbp3-24315_mouse-gprc5a-rai3-mab-gprc5a-7893-azide-and-bsa-free-2412025801633.jpg "Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) - Azide and BSA Free [NBP3-24315] -")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # GPRC5A/7893
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fragment (around aa200-357) of human GPRC5A/RAI3 protein (exact sequence is proprietary)
Localization
Cell membrane. Cytoplasmic vesicle membrane. Localized in the plasma membrane and perinuclear vesicles.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Antibody without azide - store at -20 to -80C.
Scientific Data Images for GPRC5A/RAI3 Antibody (GPRC5A/7893) - Azide and BSA Free
Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) - Azide and BSA Free [NBP3-24315] -
Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) - Azide and BSA Free [NBP3-24315] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with GPRC5A/RAI3 Antibody (GPRC5A/7893). Inset: PBS instead of primary antibody; secondary only negative control.![GPRC5A/RAI3 Antibody (GPRC5A/7893) - Azide and BSA Free Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) - Azide and BSA Free [NBP3-24315] -](https://resources.rndsystems.com/images/products/nbp3-24315_mouse-gprc5a-rai3-mab-gprc5a-7893-azide-and-bsa-free-24120257595046.jpg "Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) - Azide and BSA Free [NBP3-24315] -")
Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) - Azide and BSA Free [NBP3-24315] -
Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) - Azide and BSA Free [NBP3-24315] - Formalin-fixed, paraffin-embedded human bladder stained with GPRC5A/RAI3 Antibody (GPRC5A/7893). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.Applications for GPRC5A/RAI3 Antibody (GPRC5A/7893) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: GPRC5A
Long Name
G Protein-coupled Receptor, Family C, Group 5, Member A
Alternate Names
RAI3, RAIG1
Gene Symbol
GPRC5A
Additional GPRC5A Products
Product Documents for GPRC5A/RAI3 Antibody (GPRC5A/7893) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GPRC5A/RAI3 Antibody (GPRC5A/7893) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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