GSK J4

Tocris Bioscience | Catalog # 4594

Histone KDM inhibitor; cell permeable
Tocris Bioscience

Key Product Details

Description

Histone KDM inhibitor; cell permeable
View all Histone Demethylase Inhibitors »

Product Description

GSK J4 is a histone lysine demethylase (KDM) inhibitor; blocks demethylation of histone H3K27. Attenuates lipopolysaccharide (LPS)-induced proinflammatory cytokine production in primary human macrophages (IC50 = 9 μM for the inhibition of TNFα release). Cell permeable. Ethyl ester derivative of GSK J1.

Negative Control also available.

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details of GSK J4, a cell permeable derivative of GSK J1, please visit the GSK J1 probe summary on the SGC website.

External Portal Information

Chemicalprobes.org

is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of GSK J4 is reviewed on the chemical probes website.

Product Specifications for GSK J4

Molecular Weight

417.50

Formula

C24H27N5O2

Storage

Store at RT

Purity

≥98% (HPLC)

Chemical Name

N-[2-(2-Pyridinyl)-6-(1,2,4,5-tetrahydro-3H-3-benzazepin-3-yl)-4-pyrimidinyl]-β-alanine ethyl ester

CAS Number

1373423-53-0

PubChem ID

71729975

InChI Key

WBKCKEHGXNWYMO-UHFFFAOYSA-N

SMILES

O=C(CCNC1=NC(C2=CC=CC=N2)=NC(N3CCC(C=CC=C4)=C4CC3)=C1)OCC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 41.75 100
Ethanol 41.75 100

Preparing Stock Solutions for GSK J4

The following data is based on the product molecular weight 417.50.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.40 mL 11.98 mL 23.95 mL
5 mM 0.48 mL 2.40 mL 4.79 mL
10 mM 0.24 mL 1.20 mL 2.40 mL
50 mM 0.05 mL 0.24 mL 0.48 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 58 publications citing the usage of this product.

Product Documents for GSK J4

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GSK J4

For research use only

Related Research Areas

Epigenetics

Customer Reviews for GSK J4 (2)

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  • GSKJ4 in vivo use
    Name: Xingyao He
    Species: Mouse
    Assay Type: In Vivo
    Cell Line/Tissue: Human DIPG cell line
    Verified Customer | Posted 04/05/2019
    Treated DIPG mice with GSKJ4. Compared survival rates for GSKJ4 alone, radiation alone, GSKJ4+RT treatments.
    GSK J4 4594
  • Worked very well
    Name: Xingyao He
    Assay Type: In Vivo
    Cell Line/Tissue: SF8628FL
    Verified Customer | Posted 11/30/2018
    For animal treatments and in vitro assays.

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