Hexosaminidase A/HEXA Antibody (3F10) - BSA Free

Novus Biologicals | Catalog # NBP2-37505

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 3F10

Format

BSA Free
View all Hexosaminidase A/HEXA Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Hexosaminidase A/HEXA expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

60.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hexosaminidase A/HEXA Antibody (3F10) - BSA Free

Western Blot: Hexosaminidase A/HEXA Antibody (3F10)BSA Free [NBP2-37505]

Western Blot: Hexosaminidase A/HEXA Antibody (3F10)BSA Free [NBP2-37505]

Western Blot: Hexosaminidase A/HEXA Antibody (3F10) [NBP2-37505] - Western blot analysis using HEXA mouse mAb against L1210 (1), and HL7702 (2) cell lysate.
Flow Cytometry: Hexosaminidase A/HEXA Antibody (3F10) - BSA Free [NBP2-37505]

Flow Cytometry: Hexosaminidase A/HEXA Antibody (3F10) - BSA Free [NBP2-37505]

Flow Cytometry: Hexosaminidase A/HEXA Antibody (3F10) [NBP2-37505] - Flow cytometric analysis of HeLa cells using HEXA mouse mAb (green) and negative control (red).
Western Blot: Hexosaminidase A/HEXA Antibody (3F10)BSA Free [NBP2-37505]

Western Blot: Hexosaminidase A/HEXA Antibody (3F10)BSA Free [NBP2-37505]

Western Blot: Hexosaminidase A/HEXA Antibody (3F10) [NBP2-37505] - Western blot analysis using HEXA mAb against human HEXA (AA: 29-181) recombinant protein. (Expected MW is 43.1 kDa)
ELISA: Hexosaminidase A/HEXA Antibody (3F10) - BSA Free [NBP2-37505]

ELISA: Hexosaminidase A/HEXA Antibody (3F10) - BSA Free [NBP2-37505]

ELISA: Hexosaminidase A/HEXA Antibody (3F10) [NBP2-37505] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Hexosaminidase A/HEXA Antibody (3F10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hexosaminidase A/HEXA

HEXA encodes the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Alpha subunit gene mutations lead to Tay-Sachs disease (GM2-gangliosidosis type I). [provided by RefSeq]

Alternate Names

HEXA, TSD

Gene Symbol

HEXA

Additional Hexosaminidase A/HEXA Products

Product Documents for Hexosaminidase A/HEXA Antibody (3F10) - BSA Free

Certificate of Analysis

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Product Specific Notices for Hexosaminidase A/HEXA Antibody (3F10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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