HGD Antibody (1F1) - Azide and BSA Free

Novus Biologicals | Catalog # H00003081-M11

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F1

Format

Azide and BSA Free
View all HGD Primary Antibodies »

Product Specifications

Immunogen

HGD (AAH20792, 1 a.a. ~ 329 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSWLYRILPSVSHKPFESIDEGHVTHNWDEVDPDPNQLRWKPFEIPKASQKKVDFVSGLHTLCGAGDIKSNNGLAIHIFLCNTSMENRCFYNSDGDFLIVPQKGNLLIYTEFGKMLVQPNEICVIQRGMRFSIDVFEETRGYILEVYGVHLELPDLGPIGANGLANPRDFLIPIAWYEDRQVPGGYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLKNFMVINSVAFDHADPSIFTVLTALRRPARSSWHLRGLPMAPWHLCLNHL

Specificity

HGD - homogentisate 1,2-dioxygenase (homogentisate oxidase) (1F1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HGD Antibody (1F1) - Azide and BSA Free

Western Blot: HGD Antibody (1F1) [H00003081-M11]

Western Blot: HGD Antibody (1F1) [H00003081-M11]

Western Blot: HGD Antibody (1F1) [H00003081-M11] - Analysis of HGD expression in transfected 293T cell line by HGD monoclonal antibody (M11), clone 1F1. Lane 1: HGD transfected lysatE (50 KDa). Lane 2: Non-transfected lysate.
Immunoprecipitation: HGD Antibody (1F1) [H00003081-M11]

Immunoprecipitation: HGD Antibody (1F1) [H00003081-M11]

Immunoprecipitation: HGD Antibody (1F1) [H00003081-M11] - Analysis of HGD transfected lysate using anti-HGD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HGD rabbit polyclonal antibody.
Sandwich ELISA: HGD Antibody (1F1) [H00003081-M11] - Detection limit for recombinant GST tagged HGD is 3 ng/ml as a capture antibody.

Applications for HGD Antibody (1F1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HGD

Homogentisate 1,2-dioxygenase (HGD) gene mutations are the molecular cause of alkaptonuria, a rare hereditary disorder of the phenylalanine catabolism. The highest expression of HGD is in the prostate, small intestine, colon, and liver. The HGD gene contains 14 exons. Conflicting reports have placed the gene at 3q2, 3q13.3-q21, 3q21-q24, 3q21-q23, or 3q25-q26. [provided by RefSeq]

Alternate Names

AKU, EC 1.13.11.5, HGOFLJ94126, homogentisate 1,2-dioxygenase, homogentisate 1,2-dioxygenase (homogentisate oxidase), homogentisate oxidase, Homogentisate oxygenase, Homogentisic acid oxidase, Homogentisicase

Entrez Gene IDs

3081 (Human)

Gene Symbol

HGD

UniProt

AAH20792 (Human)

Additional HGD Products

Product Documents for HGD Antibody (1F1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HGD Antibody (1F1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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