HGS Antibody

Novus Biologicals | Catalog # NB100-55249

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all HGS Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 727 and the C-terminus (resude 777) of human growth factor-regulated tyrosine kinase substrate using the numbering given in entry NP_004703.1 (GeneID 9146).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HGS Antibody

Western Blot: HGS Antibody [NB100-55249]

Western Blot: HGS Antibody [NB100-55249]

Western Blot: HGS Antibody [NB100-55249] - Detection of Human HRS by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody NB100-55249 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: HGS Antibody [NB100-55249]

Western Blot: HGS Antibody [NB100-55249]

Western Blot: HGS Antibody [NB100-55249] - Whole cell lysate (15 ug) from Renca, CT26.WT and 4T1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: HGS Antibody [NB100-55249]

Immunoprecipitation: HGS Antibody [NB100-55249]

Immunoprecipitation: HGS Antibody [NB100-55249] - Detection of human HRS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HRS antibody NB100-55249 (lot NB100-55249-2) used for IP at 6 ug per reaction. HRS was also immunoprecipitated by a previous lot of this antibody (NB100-55249-1) and another rabbit anti-HRS antibody. For blotting immunoprecipitated HRS, NB100-55249 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for HGS Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HGS

HRS plays an esssential role in endosome membrane invagination and formation of multivesicular bodies (MVBs). It is required during gastrulation and appears to regulate early embryonic signaling pathways. HRS inhibits tyrosine kinase receptor signaling by promoting degradation of the tyrosine-phosphorylated active receptor, possibly by sorting activated receptors into MVBs. The MVBs are then trafficked to the lysosome where their contents are degraded.

Alternate Names

FLJ39878, hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, HRSFLJ11421, human growth factor-regulated tyrosine kinase substrate, Protein pp110, Vps27, ZFYVE8

Entrez Gene IDs

9146 (Human)

Gene Symbol

HGS

UniProt

O14964 (Human)

Additional HGS Products

Product Documents for HGS Antibody

Certificate of Analysis

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Product Specific Notices for HGS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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