Horse IgG, Fc Native Protein

Novus Biologicals | Catalog # NBP1-97034

Novus Biologicals

Key Product Details

Applications

Western Blot, ELISA, SDS-PAGE
View all Horse IgG, Fc Proteins and Enzymes »

Product Specifications

Description

Native Equine Horse IgG, Fc

Source: Normal serum

Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis.

Purity

Multi-step

Application Notes

This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Suggested application: IHC.

Specificity

Assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum, anti-Horse IgG and anti-Horse IgG F(c). No reaction was observed against anti-Horse IgG F(ab')2 or anti-Papain.

Protein / Peptide Type

Native Protein

Scientific Data Images for Horse IgG, Fc Native Protein

SDS-PAGE: Horse IgG, Fc Native Protein [NBP1-97034]

SDS-PAGE: Horse IgG, Fc Native Protein [NBP1-97034]

SDS-Page: Horse IgG, Fc Native Protein [NBP1-97034] - Lane 1: Horse IgG Fragment Reduced. Lane 2: Horse IgG Fragment Non reduced. Load: 1.0ug per lane.

Formulation, Preparation, and Storage

NBP1-97034
Preparation Method This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Horse IgG F(c) fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum, anti-Horse IgG and anti-Horse IgG F(c). No reaction was observed against anti-Horse IgG F(ab')2 or anti-Papain.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative 0.01% Sodium Azide
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Horse IgG, Fc

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins.

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Product Documents for Horse IgG, Fc Native Protein

Certificate of Analysis

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Download SDS

Product Specific Notices for Horse IgG, Fc Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Horse IgG, Fc Native Protein

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