PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.
HT PARP in vivo Pharmacodynamic ELISA Kit II
R&D Systems | Catalog # 4520B-096-K
The HT PARP In Vivo Pharmacodynamic ELISA Kit II (2nd Gen), 4520B-096-K, has been transitioned and replaced by a new version, 4520C-096-K, which is now available.
Discontinued Product
4520B-096-K has been discontinued.
View all PARP products.
Key Product Details
Features
Monitors in vivo PARP activity within cells based on levels of PAR.
Key Benefits
- Pre-coated 96 well capture antibody plates
- High signal to noise ratio
- Detection sensitivity of 10 pg/mL of PAR
- Broad linear dynamic range to 1,000 pg/mL
- Reduced inter-assay variability
- Validated assay that measures drug action on PARP in both in vivo and in vitro settings
- 96 test size
Species
Multi-Species
Product Summary for HT PARP in vivo Pharmacodynamic ELISA Kit II
Principle of the Assay
A monoclonal antibody specific for PAR has been pre-coated onto a microplate to capture cellular PAR and PAR attached to proteins. Incubation with a PAR Monoclonal Biotinylated Detecting Antibody, followed by addition of Streptavidin-HRP and a chemiluminescent HRP substrate yields relative light units (RLU) that directly correlate with the amount of cellular PAR.
Product Specifications
- Quantitation of PAR in tissue lysates and culture cells.
- Monitoring the efficacy of PARP inhibitors on PAR formation in vivo.
- Verifying observations of enhanced cancer cell cytotoxicity arising from PARP inhibitor/anticancer drug combination therapy.
- Facilitating development of PARP and PARG targeted therapeutics.
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Scientific Data Images for HT PARP in vivo Pharmacodynamic ELISA Kit II
Affect of PJ34 PARP Inhibitor
Sample processing can potentially generate artifactual PAR synthesis during resuspension and incubation in Cell Lysis Buffer. Methods to mitigate this effect should be carefully considered as part of the experimental design. (e.g. The addition of a PARP inhibitor to the Cell Lysis Buffer such as PJ 34 Hydrochloride (Tocris™, Catalog #3255) can help mitigate artifactual PAR synthesis).Kit Contents for HT PARP in vivo Pharmacodynamic ELISA Kit II
- PAR Standard, 25 pg/µl
- WIL2-NS Cell Lysate Control Standard, Low
- WIL2-NS Cell Lysate Control Standard, Medium
- WIL2-NS Cell Lysate Control Standard, High
- PAR Monoclonal Biotinylated Detecting Antibody
- Streptavidin-HRP
- DNase I, 2 Units/µl
- PARP Sample Buffer
- PARP Cell Lysis Reagent
- 100X Magnesium Cation
- PARP Antibody Diluent
- 20% SDS
- Pre-coated white 96-stripwell plate and 4 plate sealers
- PARP PeroxyGlow A
- PARP PeroxyGlow B
Formulation, Preparation, and Storage
Shipping
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: PARP
Long Name
Poly [ADP-ribose] Polymerase
Alternate Names
ADPRT, PARP1, PPOL
Additional PARP Products
Product Documents for HT PARP in vivo Pharmacodynamic ELISA Kit II
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Related Research Areas
Citations for HT PARP in vivo Pharmacodynamic ELISA Kit II
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