Catalog Number: 3255
Chemical Name: N-(5,6-Dihydro-6-oxo-2-phenanthridinyl)-2-acetamide hydrochloride
Biological Activity
Potent inhibitor of poly(ADP-ribose) polymerase (PARP) (EC50 = 20 nM). ~1000-fold more potent than 3-Aminobenzamide (Cat. No. 0788). Protects primary neuronal cells from oxygen-glucose deprivation in vitro and reduces infarct size following focal cerebral ischemia in vivo. Displays protective effects against cisplatin-induced kidney injury. Also displays activity at Pim-1 and Pim-2 kinases at higher concentrations (IC50 values are 3.7 and 16 μM respectively).
Technical Data
  • M.Wt:
    331.8
  • Formula:
    C17H17N3O2.HCl
  • Solubility:
    Soluble to 100 mM in water and to 100 mM in DMSO
  • Purity:
    >98%
  • Storage:
    Desiccate at RT
  • CAS No:
    344458-15-7
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Protective effects of PJ34, a novel, potent inhibitor of poly(ADP-ribose) polymerase (PARP) in in vitro and in vivo models of stroke.
    Abdelkarim et al.
    Int.J.Mol.Med., 2001;7:255
  2. Poly(ADPR)polymerase inhibition and apoptosis induction in cDDP-treated human carcinoma cell lines.
    Gambi et al.
    Biochem.Pharmacol., 2008;75:2356
  3. Poly(ADP-ribose) polymerase 1 activation is required for cisplatin nephrotoxicity.
    Kim et al.
    Kidney Int., 2012;82:193
  4. Identification of Pim kinases as novel targets for PJ34 with confounding effects in PARP biology.
    Antolin et al.
    ACS Chem.Biol., 2012;7:1962
Citations:

The citations listed below are publications that use Tocris products. Selected citations for PJ 34 hydrochloride include:

1 Citations: Showing 1 - 1

  1. Structure of human tankyrase 1 in complex with small-molecule inhibitors PJ34 and XAV939.
    Authors: Kirby Et al.
    Acta Crystallogr Sect F Struct Biol Cryst Commun 2012;68:115

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