Human ADAM19 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF5050V Datasheet / COA / SDS

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AF5050V-100UG

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Human ADAM19 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAM19 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse ADAM19 is observed, and less than 5% cross-reactivity with recombinant human (rh)&nb

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAM19
Arg26-Gln501 (Ser32 del) (Lys33Arg)
Accession # Q9H013

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ADAM19

ADAM19 (a disintegrin and metalloprotease 19; also MADDAM and Meltrin-beta ) is a 95‑100 kDa member of the M12B peptidase family of enzymes. It is expressed on multiple cell types including monocytes, fibroblasts, osteoblasts and dendritic cells. The human ADAM19 proenzyme is a 931 amino acid (aa) type I transmembrane protein. It contains a 178 aa cleavable proregion (aa 26‑203) and a 497 aa extracellular domain (ECD) (aa 204‑702). The ECD contains a metalloprotease domain (aa 211‑409), a disintegrin region (aa 417‑503) and a Cys-rich domain (aa 504‑650). The ECD circulates in plasma bound to 700 kDa alpha 2-Macroglobulin. One splice variant shows an alternate start site at Met71, while two isoforms show variations after Lys902. Over aa 205‑702, human ADAM19 is 87% aa identical to mouse ADAM19.

Long Name

A Disintegrin and Metalloprotease-like Domain 19

Entrez Gene IDs

8728 (Human); 11492 (Mouse)

Alternate Names

a disintegrin and metalloproteinase domain 19 (meltrin beta); ADAM 19; ADAM metallopeptidase domain 19; ADAM19; disintegrin and metalloproteinase domain-containing protein 19; EC 3.4.24; EC 3.4.24.-; MADDAM; Meltrin beta; meltrin-beta; Metalloprotease and disintegrin dendritic antigen marker; MLTNBmeltrin beta