Human ADP-Sugar Pyrophosphatase/NUDT5 Alexa Fluor® 405-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: ADP-Sugar Pyrophosphatase/NUDT5

ADP‑Sugar Pyrophosphatase (NUDT-5, Nucleoside diphosphate-linked moiety X motif 5; also YSA1H and 8-oxo-dGDP phosphatase) is a member of the Nudix hydrolase family of proteins. Although the predicted MW of NUDT-5 is 24 kDa, it runs anomalously at 32-36 kDa in SDS-Page. NUDT-5 is a widely expressed intracellular homodimer that has at least two functions. First, it possesses pyrophosphatase activity that prevents nonenzymatic ribosylation of proteins, and second, it hydrolyzes 8-oxoGDP (plus 8-OH-dADP), thus removing an oxidized nucleoside from the nucleotide pool and its potential for incorporation into either DNA or RNA. Such incorporation likely results in transcriptional or translational errors. Human NUDT-5 is 219 amino acids (aa) in length (SwissProt #:Q9UKK9). It is contains an N-terminal substrate binding region (aa 28-51), a Nudix hydrolase domain (aa 57-197) and an extended C-terminal a-helix (aa 211-219) NUDT-5 is acetylated on Met1, Lys42, Lys210, and Lys218, and phosphorylated on Tyr74. There are two isoform variants, one that shows a deletion of aa 163-165, and another that contains a 13 aa substitution for aa 164-219. Full-length human and mouse NUDT-5 share 82% aa sequence identity.

Product Datasheets

Related Research Areas

• Phosphatases and Regulators
• Search Kinases and Phosphatases