Human ADP-Sugar Pyrophosphatase/NUDT5 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human ADP‑Sugar Pyrophosphatase/NUDT5 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human ADP‑Sugar Pyrophosphatase/NUDT5
    Glu2-Phe219
    Accession # Q9UKK9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human ADP‑Sugar Pyrophosphatase/
NUDT5 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and human liver tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ADP‑Sugar Pyrophosphatase/NUDT5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6414) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADP‑Sugar Pyrophosphatase/NUDT5 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADP-Sugar Pyrophosphatase/NUDT5
ADP‑Sugar Pyrophosphatase (NUDT-5, Nucleoside diphosphate-linked moiety X motif 5; also YSA1H and 8-oxo-dGDP phosphatase) is a member of the Nudix hydrolase family of proteins. Although the predicted MW of NUDT-5 is 24 kDa, it runs anomalously at 32-36 kDa in SDS-Page. NUDT-5 is a widely expressed intracellular homodimer that has at least two functions. First, it possesses pyrophosphatase activity that prevents nonenzymatic ribosylation of proteins, and second, it hydrolyzes 8-oxoGDP (plus 8-OH-dADP), thus removing an oxidized nucleoside from the nucleotide pool and its potential for incorporation into either DNA or RNA. Such incorporation likely results in transcriptional or translational errors. Human NUDT-5 is 219 amino acids (aa) in length (SwissProt #:Q9UKK9). It is contains an N-terminal substrate binding region (aa 28-51), a Nudix hydrolase domain (aa 57-197) and an extended C-terminal a-helix (aa 211-219) NUDT-5 is acetylated on Met1, Lys42, Lys210, and Lys218, and phosphorylated on Tyr74. There are two isoform variants, one that shows a deletion of aa 163-165, and another that contains a 13 aa substitution for aa 164-219. Full-length human and mouse NUDT-5 share 82% aa sequence identity.

  • Entrez Gene IDs:
    11164 (Human)
  • Alternate Names:
    EC 3.6.1.-; EC 3.6.1.13; hYSAH1; nucleoside diphosphate linked moiety X-type motif 5; Nucleoside diphosphate-linked moiety X motif 5; nudix (nucleoside diphosphate linked moiety X)-type motif 5; Nudix motif 5; NUDT5; YSA1; YSA1H; YSA1HADP-sugar pyrophosphatase
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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