Human AMIGO Alexa Fluor® 532-conjugated Antibody

Catalog #: AF2565X Datasheet / COA / SDS

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AF2565X-100UG

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Human AMIGO Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human AMIGO in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) AMIGO2 is observed and less than 1% cross-reactivity with rhAMIGO3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human AMIGO
Met1-Gly365
Accession # Q86WK6

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: AMIGO

AMIGO is a 65 kDa type I transmembrane glycoprotein that belongs to both the Ig- and LLR (leucine-rich repeat)- superfamilies. It is expressed on developing neuronal fiber tracts and participates in their formation. AMIGO forms both homophilic and heterophilic complexes with AMIGO2 and 3, suggesting an adhesive function for the molecule. Human AMIGO extracellular domain is 87% amino acid identical to mouse AMIGO, and 40% identical to human AMIGO2 and 3.

Long Name

Amphoterin-induced Gene and ORF

Entrez Gene IDs

57463 (Human); 229715 (Mouse)

Alternate Names

adhesion molecule with Ig-like domain 1; ali2; Alivin-2; AMIGO; AMIGO1; AMIGO-1; AMIGOamphoterin-induced protein 1; amphoterin-induced gene and ORF; KIAA1163