Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all AMIGO Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human AMIGO
Met1-Gly365
Accession # Q86WK6

Specificity

Detects human AMIGO in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) AMIGO2 is observed and less than 1% cross-reactivity with rhAMIGO3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human AMIGO Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human AMIGO

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AMIGO

AMIGO is a 65 kDa type I transmembrane glycoprotein that belongs to both the Ig- and LLR (leucine-rich repeat)- superfamilies. It is expressed on developing neuronal fiber tracts and participates in their formation. AMIGO forms both homophilic and heterophilic complexes with AMIGO2 and 3, suggesting an adhesive function for the molecule. Human AMIGO extracellular domain is 87% amino acid identical to mouse AMIGO, and 40% identical to human AMIGO2 and 3.

Long Name

Amphoterin-induced Gene and ORF

Alternate Names

ali2, Alivin-2, AMIGO1

Entrez Gene IDs

57463 (Human); 229715 (Mouse)

Gene Symbol

AMIGO1

UniProt

Q86WK6

Additional AMIGO Products

Product Documents for Human AMIGO Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human AMIGO Antibody

For research use only

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FAQs

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