Human Aminopeptidase P1/XPNPEP1 Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB2970X Datasheet / COA / SDS

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FAB2970X-100UG

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Human Aminopeptidase P1/XPNPEP1 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Aminopeptidase P1/XPNPEP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) XPNPEP1, rmXPNPEP2, or recombinant human XPNPEP2 is observed.

Source

Monoclonal Mouse IgG1 Clone # 409111

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Aminopeptidase P1/XPNPEP1
Met1-Gln622
Accession # Q9NQW7

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Aminopeptidase P1/XPNPEP1

The human XPNPEP1 gene encodes aminopeptidase P1 (APP1), which is also known as X-prolyl aminopeptidase with gene aliases of SAMP, XPNPEP, XPNPEPL, and XPNPEPL1 (1‑3). It is a member of the M24 family of metalloproteases, which also contains methionine aminopeptidases, X-Pro dipeptidase, aminopeptidase P2, aminopeptidase P homolog, proliferation-associated protein 1, and suppressor of Ty homolog or chromatin-specific transcription elongation factor large subunit (4). It is a soluble enzyme, in contrast to the GPI-anchored APP2 encoded by XPNPEP2 (5). Human APP1 is widely expressed (3). APP1 removes a N-terminal amino acid from a peptide that contains a Pro residue at the second position. The amino acid sequence of human APP1 is 99%, 97%, 95%, 74%, and 73% identical to that of canine, bovine, mouse/rat, Xenopus, and zebrafish, respectively.

Entrez Gene IDs

7511 (Human)

Alternate Names

Aminoacylproline aminopeptidase; Aminopeptidase P1; APP1; Cytosolic aminopeptidase P; cytosolic; EC 3.4.11.9; SAMP; xaa-Pro aminopeptidase 1; XPNPEP1; XPNPEPLX-prolyl aminopeptidase (aminopeptidase P)-like; X-prolyl aminopeptidase (aminopeptidase P) 1, soluble; X-prolyl aminopeptidase 1, soluble