Human Cadherin-12 Alexa Fluor® 647-conjugated Antibody

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FAB2240R-100UG
R&D Systems Antibodies
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Human Cadherin-12 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cadherin‑12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cadherin-8, -11, -13, -17, rhE-Cadherin, rhP-Cadherin, rhR-Cadherin, or rhVR-Cadherin is observed.
Source
Monoclonal Rat IgG1 Clone # 343621
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cadherin-12
Met1-Pro609
Accession # P55289
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells

Human neural progenitor cells differentiated by growth factor withdrawal

 

Note: Since classic Cadherins can be protected from trypsin treatment in the presence of Ca2+, cells in monolayer cultures are harvested with 0.01% Trypsin in the presence of 1-5 mM CaCl2 at 37° C. Flow cytometry can be performed according to the standard procedures, except that all the cell staining and washing steps are performed in the presence of Ca2+ and Mg2+ (e.g. using FACS buffer: PBS containing 1 mM CaCl2, 1 mM MgCl2, 2% FBS and 0.02% sodium azide).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Cadherin-12

The cadherin superfamily is a large family of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least four subfamilies based on its member’s extracellular (EC) regions and cytoplasmic domains (1, 2). These include classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules that contain a variable number of EC and transmembrane (TM) domains (1).
Cadherin‑12, also known as brain-cadherin and N-cadherin 2, is a 150 kDa classical cadherin. Classical family molecules are modular in their extracellular region, mediating calcium-dependent cell-cell adhesion through their five EC Ca++-binding repeats (2). Cadherin-12 can be further identified as a type II classical cadherin, due to the absence of a His-Ala-Val motif in its most N-terminal cadherin repeat (3). Human Cadherin-12 is synthesized as a 794 amino acid (aa) type I transmembrane preproprotein that contains a 23 aa signal peptide, a 31 aa prosequence, a 555 aa extracellular region, a 28 aa transmembrane segment, and a 157 aa cytoplasmic domain (4, 5). The five EC cadherin domains are approximately 110 aa in length and generate two beta -sheets that are oriented like bread in a sandwich. Human Cadherin-12 EC region is 96% aa identical to mouse Cadherin-12 EC region. Cadherin-12 is expressed specifically in CNS neurons. The bulk of its expression is postnatal, and it is proposed to be involved in synaptogenesis (4). As a classic cadherin, Cadherin-12 will form homodimers and promote intercellular adhesion with itself and, possibly, cadherins-8 and -14 (6).

References
  1. Koch, A.W. et al. (2004) Cell. Mol. Life Sci. 61:1884.
  2. Angst, B.D. et al. (2001) J. Cell Sci. 114:629.
  3. Gessner, R. and R. Tauber (2000) Ann. N.Y. Acad. Sci. 915:136.
  4. Selig, S. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2398.
  5. Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15.
  6. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
Entrez Gene IDs
1010 (Human)
Alternate Names
Br-Cadherin; cadherin 12, type 2 (N-cadherin 2); Cadherin12; Cadherin-12; CDH12; CDHB; FLJ34857; N-Cadherin 2; Neural type cadherin 2; neural type, 2; neuronal cadherin 2

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