Home / Carbonic Anhydrase I/CA1 / Human Carbonic Anhydrase I/CA1 Alexa Fluor® 700-conjugated Antibody

Human Carbonic Anhydrase I/CA1 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB2180N Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2180N-100UG
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbonic Anhydrase I/CA1 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carbonic Anhydrase I/CA1 in direct ELISAs and Western blots. In Western blots, 30% cross-reactivity with recombinant human (rh) CA2 is observed and no cross-reactivity with rhCA3, -4, -7, -8, -10, -11, -12, -13, or -14 is observed.
Source
Monoclonal Rat IgG2a Clone # 363121
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human Carbonic Anhydrase I/CA1
Ala2-Phe261
Accession # P00915
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Carbonic Anhydrase I/CA1

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). CA1 is a cytosolic enzyme with the highest levels in erythrocytes and is a very early marker for erythroid differentiation (2). The activity of CA1 can also be measured by its ability to catalyze the reaction CO2 + H2O → HCO3- + H+, using a published method (3).

Entrez Gene IDs
759 (Human)
Alternate Names
CA1; CA-I; Car1; Carbonate dehydratase I; carbonic anhydrase 1; Carbonic anhydrase B; Carbonic Anhydrase I; carbonic anhydrase ICAB; carbonic dehydratase; EC 4.2.1.1

Product Datasheets

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