Detects human Carbonic Anhydrase I/CA1 in direct ELISAs and Western blots. In Western blots, 30% cross-reactivity with recombinant human (rh) CA2 is observed and no cross-reactivity with rhCA3, -4, -7, -8, -10, -11, -12, -13, or -14 is observed.
Monoclonal Rat IgG2A Clone # 363121
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Carbonic Anhydrase I/CA1 Ala2-Phe261 Accession # P00915
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Carbonic Anhydrase I/CA1 by Western Blot. Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line, human liver tissue, and human colon tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Carbonic Anhydrase I/CA1 Monoclonal Antibody (Catalog # MAB2180) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase I/CA1
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). CA1 is a cytosolic enzyme with the highest levels in erythrocytes and is a very early marker for erythroid differentiation (2). The activity of CA1 can also be measured by its ability to catalyze the reaction CO2 + H2O → HCO3- + H+, using a published method (3).
Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.
Wilbur, K.M. and N.G. Anderson (1948) J. Biol. Chem. 176:147.
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