Human Carbonic Anhydrase XIV/CA14 Alexa Fluor™ Plus 555‑conjugated Antibody

R&D Systems | Catalog # FAB2195AFP555

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 312908
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Product Specifications

Specificity

Detects human Carbonic Anhydrase XIV/CA14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross‑reactivity with recombinant mouse CA14, 10% cross‑reactivity with recombinant human (rh) CA5A, and no cross-reactivity with rhCA1, 2, 3, 4, 6, 7, 8, 9, 10, 12, or 13 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications

Application
Recommended Usage

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Carbonic Anhydrase XIV/CA14

Carbonic Anhydrase 14 (CA14) has a C-terminal transmembrane domain (aa 291 - 311) and a short cytoplasmic tail (aa 312 - 337). As a functioning enzyme of the CA family, it catalyzes the reversible hydration of CO2. CA14 is highly expressed in all parts of the central nervous system with lower expression in adult liver, heart, small intestine, colon, kidney, urinary bladder and skeletal muscle. The rhCA14 corresponds to the 272 aa ectodomain that has a predicted molecular mass of approximately 32 kDa and an apparent molecular mass of approximately 43 kDa in SDS-PAGE. The amino acid sequence of the human ectodomain shares 98%, 86%, 84%, and 83% identity with that of chimpanzee, bovine/dog, rat and mouse, respectively.

Alternate Names

CA14

Entrez Gene IDs

23632 (Human); 23831 (Mouse)

Gene Symbol

CA14

UniProt

Additional Carbonic Anhydrase XIV/CA14 Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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