Human CD6 PE‑conjugated Antibody

R&D Systems | Catalog # FAB627P

R&D Systems
Discontinued Product
FAB627P has been discontinued. View all CD6 products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Polyclonal Goat IgG
View all CD6 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD6
His18-Glu398
Accession # Q8WWJ7

Specificity

Detects recombinant human CD6 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CD6 PE‑conjugated Antibody

Detection of CD6 antibody in Human Whole Blood Lymphocytes antibody by Flow Cytometry.

Detection of CD6 in Human Whole Blood Lymphocytes by Flow Cytometry.

Human whole blood lymphocytes were stained with Goat Anti-Human CD6 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB627P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD6 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human whole blood lymphocytes

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD6

Human CD6 is a 105 kDa (unphosphorylated) -130 kDa (hyperphosphorylated) glycoprotein Group B member of the SRCR (Scavenger Receptor Cys-Rich) superfamily of molecules. It has a restricted expression pattern, being limited to select CD56dim NK cells, B1a B cells (IgM producers), thymocytes and virtually all resting T cells. Mature CD6 is a type I transmembrane protein 651 amino acids (aa) in length. It contains a 385 aa extracellular region (aa 19-402) that possesses three SRCR repeats, and a relatively long 245 aa cytoplasmic domain. There are at least six alternative splice forms for human CD6, four of which (plus the full-length form) contain an unspliced extracellular region. The isoform used here for immunization (SwissProt: Q8WWJ7) is one of these four, showing splicing only in the cytoplasmic region. Thus, the polyclonal antibody used here will recognize all full-length CD6 molecules, and may, by an absence of staining, suggest the presence of the two isoform variants that are missing the third SRCR repeat that lies between aa 259-361. CD6 has multiple interaction partners. It complexes with CD5 in cis, contributing to the immunological synapse; in trans, it binds to dendritic cell CD166/ALCAM on the surface of adjacent cells. CD6 is also known to bind soluble galectin-1 and -3, and to bind the PAMPs associated with pathogenic microbes. Notably, on T cells and following activation, full-length CD6 is downregulated, and a splice variant lacking the third SRCR is upregulated, abrogating any CD6:CD166 interaction. Functions attributed to CD6 include the induction of chemokine secretion by NK cells, costimulation of antigen recognition by T cells, and an antimicrobial activity attributed to an 85 kDa soluble form of CD6. Over aa 18-398, human and mouse CD6 share 68% aa sequence identity.

Alternate Names

CD6

Entrez Gene IDs

923 (Human); 12511 (Mouse); 102143944 (Cynomolgus Monkey)

Gene Symbol

CD6

UniProt

Q8WWJ7

Additional CD6 Products

Product Documents for Human CD6 PE‑conjugated Antibody

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Product Specific Notices for Human CD6 PE‑conjugated Antibody

For research use only

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