Human Chitinase 3-like 1 Quantikine ELISA Kit

  (16 citations)     
Datasheet / CoA / SDS
Product Details
Assay Procedure
Citations (16)
FAQs
Supplemental Products
Reviews
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)
  • Sensitivity
    8.15 pg/mL
  • Assay Range
    62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Natural and recombinant human CHI3L1
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC63 , Quantikine Immunoassay Control Set 841 for Human CHI3L1 - Please Inquire
Product Summary
The Quantikine Human CHI3L1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure CHI3L1 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human CHI3L1 and antibodies raised against recombinant human CHI3L1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural CHI3L1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human CHI3L1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 566 1031 2202 606 1019 2212
Standard Deviation 26.7 44.4 102.6 32.1 59.1 153.1
CV% 4.7 4.3 4.7 5.3 5.8 6.9

Recovery

The recovery of CHI3L1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 93-100
Urine (n=4) 105 90-115
Linearity
To assess the linearity of the assay, samples containing high concentrations of CHI3L1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 Chitinase 3-like 1 [HRP]
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Chitinase 3-like 1
Chitinase 3-like 1 (CHI3L1), also called BRP39 in mouse or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family although it does not show chitotriosidase activity. CHI3L1 is expressed by articular chondrocytes, synovial cells, activated monocyte-derived macrophages, neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. CHI3L1 binds to chitin and heparins, and it enhances cell adhesion, proliferation and tumor angiogenesis. Circulating CHI3L1 is elevated during inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. It is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary.
  • Entrez Gene IDs:
    1116 (Human); 12654 (Mouse)
  • Alternate Names:
    AW208766; Brp39; CHI3L1; Chitinase 3 like 1; Chitinase 3-like 1; gp39; HCgp39; YKL-40
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
Filter your results:

Species
Sample Type
  1. Chitinase-3-Like Protein 1 (YKL-40) Reflects the Severity of Symptoms in Atopic Dermatitis
    Authors: J Salomon, ? Matusiak, D Nowicka-Su, JC Szepietows
    J Immunol Res, 2017;2017(0):5746031.
    Species: Human
    Sample Type: Serum
  2. Effects of surgery and propofol-remifentanil total intravenous anesthesia on cerebrospinal fluid biomarkers of inflammation, Alzheimer's disease, and neuronal injury in humans: a cohort study
    Authors: A Pikwer, M Castegren, S Namdar, K Blennow, H Zetterberg, N Mattsson
    J Neuroinflammation, 2017;14(1):193.
    Species: Human
    Sample Type: CSF
  3. Effect of physical exercise on markers of neuronal dysfunction in cerebrospinal fluid in patients with Alzheimer's disease
    Authors: CS Jensen, E Portelius, P Høgh, L Wermuth, K Blennow, H Zetterberg, SG Hasselbalc, AH Simonsen
    Alzheimers Dement (N Y), 2017;3(2):284-290.
    Species: Human
    Sample Type: CSF
  4. Chitinase-3-Like Protein 1 (YKL-40) Is a New Biomarker of Inflammation in Psoriasis
    Authors: J Salomon, ? Matusiak, D Nowicka-Su, JC Szepietows
    Mediators Inflamm., 2017;2017(0):9538451.
    Species: Human
    Sample Type: Serum
  5. Diagnosis of cardiac surgery-associated acute kidney injury: differential roles of creatinine, chitinase 3-like protein 1 and neutrophil gelatinase-associated lipocalin: a prospective cohort study
    Authors: J De Loor, I Herck, K Francois, A Van Wesema, L Nuytinck, E Meyer, EA Hoste
    Ann Intensive Care, 2017;7(1):24.
    Species: Human
    Sample Type: Urine
  6. Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques
    Authors: SR Langley, K Willeit, A Didangelos, LP Matic, P Skroblin, J Barallobre, M Lengquist, G Rungger, A Kapustin, L Kedenko, C Molenaar, R Lu, T Barwari, G Suna, X Yin, B Iglseder, B Paulweber, P Willeit, J Shalhoub, G Pasterkamp, AH Davies, C Monaco, U Hedin, CM Shanahan, J Willeit, S Kiechl, M Mayr
    J. Clin. Invest, 2017;0(0):.
    Species: Human
    Sample Type: Plasma
  7. Cerebrospinal Fluid Biomarkers of Neurodegeneration Are Decreased or Normal in Narcolepsy
    Authors: P Jørgen Jen, L Østergaard, JM Czarna Bah, S Modvig, K Fog, A Holm, B Rahbek Kor, S Gammeltoft
    Sleep, 2017;40(1):.
    Species: Human
    Sample Type: CSF
  8. MiR-205 and MiR-373 Are Associated with Aggressive Human Mucinous Colorectal Cancer
    Authors: Annette Eyking
    PLoS ONE, 2016;11(6):e0156871.
    Species: Human
    Sample Type: Cell Culture Supernates
  9. The impact of acute aerobic exercise on chitinase 3-like protein 1 and intelectin-1 expression in obesity
    Exp. Biol. Med. (Maywood), 2016;241(2):216-21.
    Species: Human
    Sample Type: Plasma
  10. Least absolute shrinkage and selection operator type methods for the identification of serum biomarkers of overweight and obesity: simulation and application
    BMC Med Res Methodol, 2016;16(1):154.
    Species: Human
    Sample Type: Serum
  11. Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis
    JCI Insight, 2016;1(20):e90954.
    Species: Human
    Sample Type: Cell Culture Supernates
  12. Cerebrospinal fluid microglia and neurodegenerative markers in twins concordant and discordant for psychotic disorders
    Authors: Viktoria Johansson
    Eur Arch Psychiatry Clin Neurosci, 2016;0(0):.
    Species: Human
    Sample Type: CSF
  13. Airway, but not serum or urinary, levels of YKL-40 reflect inflammation in early cystic fibrosis lung disease.
    Authors: Fantino E, Gangell C, Hartl D, Sly P
    BMC Pulm Med, 2014;14(0):28.
    Species: Human
    Sample Type: BALF
  14. Stat3 downstream gene product chitinase 3-like 1 is a potential biomarker of inflammation-induced lung cancer in multiple mouse lung tumor models and humans.
    Authors: Yan, Cong, Ding, Xinchun, Wu, Lingyan, Yu, Menggang, Qu, Peng, Du, Hong
    PLoS ONE, 2013;8(4):e61984.
    Species: Human
    Sample Type: Serum
  15. Levels of YKL-40 in pleural effusions and blood from patients with pulmonary or pleural disease.
    Authors: Kim HR, Jun CD, Lee KS, Cho JH, Jeong ET, Yang SH, Lee YJ, Park DS
    Cytokine, 2012;58(3):336-43.
    Species: Human
    Sample Type: Serum
  16. The chitinase-like protein YKL-40 modulates cystic fibrosis lung disease.
    Authors: Hector A, Kormann MS, Mack I, Latzin P, Casaulta C, Kieninger E, Zhou Z, Yildirim AÖ, Bohla A, Rieber N, Kappler M, Koller B, Eber E, Eickmeier O, Zielen S, Eickelberg O, Griese M, Mall MA, Hartl D
    PLoS ONE, 2011;6(9):e24399.
    Species: Human
    Sample Type: Serum
Expand to show all Citations

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
ELISA Controls
Description Application Cat# Citations Images  

Quantikine Immunoassay Control Set 841 for Human CHI3L1

Ctrl QC63
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental ELISA Products
Description Application Cat# Citations Images  

Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human Chitinase 3-like 1 Quantikine ELISA Kit?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
R&D Systems Guarantee