Human Chitinase 3-like 1 Quantikine ELISA Kit

(9 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)
  • Sensitivity
    8.15 pg/mL
  • Assay Range
    62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Natural and recombinant human CHI3L1
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC63, Quantikine Immunoassay Control Set 841 for Human CHI3L1 - Please Inquire
Product Summary
The Quantikine Human CHI3L1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure CHI3L1 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human CHI3L1 and antibodies raised against recombinant human CHI3L1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural CHI3L1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human CHI3L1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean5661031220260610192212
Standard Deviation26.744.4102.632.159.1153.1
CV%4.74.34.75.35.86.9

Recovery

The recovery of CHI3L1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 93-100
Urine (n=4) 105 90-115
Linearity
To assess the linearity of the assay, samples containing high concentrations of CHI3L1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Chitinase 3-like 1 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Chitinase 3-like 1
Chitinase 3-like 1 (CHI3L1), also called BRP39 in mouse or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family although it does not show chitotriosidase activity. CHI3L1 is expressed by articular chondrocytes, synovial cells, activated monocyte-derived macrophages, neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. CHI3L1 binds to chitin and heparins, and it enhances cell adhesion, proliferation and tumor angiogenesis. Circulating CHI3L1 is elevated during inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. It is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary.
    • Entrez Gene IDs
      1116 (Human); 12654 (Mouse);
    • Alternate Names
      AW208766; Brp39; CHI3L1; Chitinase 3 like 1; Chitinase 3-like 1; gp39; HCgp39; YKL-40;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    9 Citations: Showing 1 - 9
    Filter your results:

    Species
    Sample Type
    1. Chitinase-3-Like Protein 1 (YKL-40) Reflects the Severity of Symptoms in Atopic Dermatitis
      Authors: J Salomon, ? Matusiak, D Nowicka-Su, JC Szepietows
      J Immunol Res, 2017;2017(0):5746031.
      Species: Human
      Sample Type: Serum
    2. MiR-205 and MiR-373 Are Associated with Aggressive Human Mucinous Colorectal Cancer
      Authors: Annette Eyking
      PLoS ONE, 2016;11(6):e0156871.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. The impact of acute aerobic exercise on chitinase 3-like protein 1 and intelectin-1 expression in obesity
      Exp. Biol. Med. (Maywood), 2016;241(2):216-21.
      Species: Human
      Sample Type: Plasma
    4. Least absolute shrinkage and selection operator type methods for the identification of serum biomarkers of overweight and obesity: simulation and application
      BMC Med Res Methodol, 2016;16(1):154.
      Species: Human
      Sample Type: Serum
    5. Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis
      JCI Insight, 2016;1(20):e90954.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Airway, but not serum or urinary, levels of YKL-40 reflect inflammation in early cystic fibrosis lung disease.
      Authors: Fantino E, Gangell C, Hartl D, Sly P
      BMC Pulm Med, 2014;14(0):28.
      Species: Human
      Sample Type: BALF
    7. Stat3 downstream gene product chitinase 3-like 1 is a potential biomarker of inflammation-induced lung cancer in multiple mouse lung tumor models and humans.
      Authors: Yan, Cong, Ding, Xinchun, Wu, Lingyan, Yu, Menggang, Qu, Peng, Du, Hong
      PLoS ONE, 2013;8(4):e61984.
      Species: Human
      Sample Type: Serum
    8. Levels of YKL-40 in pleural effusions and blood from patients with pulmonary or pleural disease.
      Authors: Kim HR, Jun CD, Lee KS, Cho JH, Jeong ET, Yang SH, Lee YJ, Park DS
      Cytokine, 2012;58(3):336-43.
      Species: Human
      Sample Type: Serum
    9. The chitinase-like protein YKL-40 modulates cystic fibrosis lung disease.
      Authors: Hector A, Kormann MS, Mack I, Latzin P, Casaulta C, Kieninger E, Zhou Z, Yildirim AÖ, Bohla A, Rieber N, Kappler M, Koller B, Eber E, Eickmeier O, Zielen S, Eickelberg O, Griese M, Mall MA, Hartl D
      PLoS ONE, 2011;6(9):e24399.
      Species: Human
      Sample Type: Serum
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 841 for Human CHI3L1

    Ctrl QC63
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....

    Have you used Human Chitinase 3-like 1 Quantikine ELISA Kit?

    Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Order Details

    Contact Information

    R&D Systems Guarantee

    Feedback

    Customer Information