Wash Buffer Concentrate, 25X

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Wash Buffer Concentrate
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Wash Buffer Concentrate, 25X Summary


Wash Buffer Concentrate (Part # 895126) contains 5 vials (100 mL/vial) of a 25X concentrated solution of buffered surfactant with preservative.  The concentrate can be diluted to a working concentration and used for the complete removal of any unbound sample or conjugate in the wells of an assay.


Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. If crystals have formed in the concentrate, warm to room temperature and mix gently until the crystals have gone into solution. May turn yellow over time.


Dilute 20 mL of Wash Buffer Concentrate to a final volume of 500 mL in deionized water. Diluted Wash Buffer may be used for up to 30 days when stored at 2-8 °C.


  • Caution: Wash Buffer Concentrate contains ProClin® which may cause an allergic reaction. Avoid breathing mist. Wear protective gloves, clothing, and eye and face protection. Wash hands thoroughly after handling. Please refer to the MSDS on our website prior to use.


Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

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Citations for Wash Buffer Concentrate, 25X

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Effect of Expansion Media on Functional Characteristics of Bone Marrow-Derived Mesenchymal Stromal Cells
    Authors: Jakl, V;Popp, T;Haupt, J;Port, M;Roesler, R;Wiese, S;Friemert, B;Rojewski, MT;Schrezenmeier, H;
    Cells  2023-08-19
  2. Increased neutralization potency and breadth elicited by a SARS-CoV-2 mRNA vaccine forming virus-like particles
    Authors: Zhang, P;Falcone, S;Tsybovsky, Y;Singh, M;Gopan, V;Miao, H;Seo, Y;Rogers, D;Renzi, I;Lai, YT;Narayanan, E;Stewart-Jones, G;Himansu, S;Carfi, A;Fauci, AS;Lusso, P;
    Proceedings of the National Academy of Sciences of the United States of America  2023-07-18
  3. Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion
    Authors: X Sun, B Wu, HC Chiang, H Deng, X Zhang, W Xiong, J Liu, AM Rozeboom, BT Harris, E Blommaert, A Gomez, RE Garcia, Y Zhou, P Mitra, M Prevost, D Zhang, D Banik, C Isaacs, D Berry, C Lai, K Chaldekas, PS Latham, CA Brantner, A Popratilof, VX Jin, N Zhang, Y Hu, MA Pujana, TJ Curiel, Z An, R Li
    Nature, 2021-11-03;0(0):.  2021-11-03
  4. Stabilization of surface-bound antibodies for ELISA based on a reversable zeolitic imidazolate framework-8 coating
    Authors: L Kang, S Smith, C Wang
    Journal of colloid and interface science, 2020-12-24;588(0):101-109.  2020-12-24
  5. Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo
    Authors: M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk
    Int J Nanomedicine, 2019-08-01;14(0):6117-6131.  2019-08-01
  6. Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation
    Authors: S Liu, J Han, H Gong, Y Li, X Bao, J Qi, H Liu, J Chen, X Wu, Y Xu, S Ma, D Wu
    Sci Rep, 2018-07-09;8(1):10328.  2018-07-09
  7. Clinical validation of a blood-based classifier for diagnostic evaluation of asymptomatic individuals with pulmonary nodules
    Authors: CE Birse, JL Tomic, HI Pass, WN Rom, RJ Lagier
    Clin Proteomics, 2017-07-05;14(0):25.  2017-07-05
  8. Optofluidic laser for dual-mode sensitive biomolecular detection with a large dynamic range.
    Authors: Wu X, Oo M, Reddy K, Chen Q, Sun Y, Fan X
    Nat Commun, 2014-04-30;5(0):3779.  2014-04-30
  9. Apolipoprotein B binding domains: evidence that they are cell-penetrating peptides that efficiently deliver antigenic peptide for cross-presentation of cytotoxic T cells.
    Authors: Sakamoto N, Rosenberg AS
    J. Immunol., 2011-03-14;186(8):5004-11.  2011-03-14
  10. Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
    Authors: Khankin EV, Mutter WP, Tamez H
    PLoS ONE, 2010-02-16;5(2):e9246.  2010-02-16
  11. First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
    Authors: Thadhani R, Powe CE, Tjoa ML
    Diabetes Care, 2009-12-10;33(3):664-9.  2009-12-10
  12. Embryonic stem cells cultured in serum-free medium acquire bovine apolipoprotein B-100 from feeder cell layers and serum replacement medium.
    Authors: Hisamatsu-Sakamoto M, Sakamoto N, Rosenberg AS
    Stem Cells, 2007-10-18;26(1):72-8.  2007-10-18
  13. Soluble triggering receptor expressed on myeloid cell-1 is increased in patients with ventilator-associated pneumonia: a preliminary report.
    Authors: Horonenko G, Hoyt JC, Robbins RA, Singarajah CU, Umar A, Pattengill J, Hayden JM
    Chest, 2007-05-15;132(1):58-63.  2007-05-15


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Reviews for Wash Buffer Concentrate, 25X

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Wash Buffer Concentrate, 25X
By Anonymous on 05/25/2024

Wash Buffer Concentrate
By Anonymous on 05/22/2024

Quantikine ELISA Wash Buffer 1
By Anonymous on 02/07/2023

Quantikine ELISA Wash Buffer 1
By Anonymous on 12/03/2021

Stable and used for long term

Quantikine ELISA Wash Buffer 1
By Hon Wai Koon on 12/01/2020

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By Gagandeep Kaur on 11/09/2020

Quantikine ELISA Wash Buffer 1
By Anonymous on 08/17/2020

Quantikine ELISA Wash Buffer 1
By Anonymous on 04/07/2020

Quantikine ELISA Wash Buffer 1
By Danlee Enzler on 04/18/2018

Quantikine ELISA Wash Buffer 1
By Leslie Priddy on 03/30/2018

Quantikine ELISA Wash Buffer 1
By Anonymous on 12/20/2017

Very easy to use it.

Quantikine Wash Buffer 1
By Anonymous on 07/19/2017

Using wash buffer completely removes any background