Human Claudin-10b Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4279R-100UG
R&D Systems Antibodies
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Human Claudin-10b Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Claudin‑10b. Stains human Claudin‑10b transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2b Clone # 394513
Immunogen
NS0 mouse myeloma cell line transfected with human Claudin‑10 b Isoform
Met1-Val228
Accession # P78369
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
HepG2 human hepatocellular carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Claudin-10b

Claudin-10 is a 228 amino acid multipass membrane protein in the Claudin family of epithelial tight junction proteins. Two splice forms of human Claudin-10 have been described with a divergent N-terminal extracellular loop. Claudin-10b (Swiss-Prot isoform 1) is relatively cation-permeant compared to Claudin-10a and is widely expressed, while Claudin‑10a is restricted to the kidney cortex. Claudin-10 is overexpressed in some carcinomas. Human Claudin‑10b shares 94% aa sequence identity with mouse and rat Claudin-10.

Entrez Gene IDs
9071 (Human); 58187 (Mouse); 290485 (Rat)
Alternate Names
Claudin10b; Claudin-10b; CLDN10; CPETRL3; OSP-L

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