Human Claudin-6 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3656R-100UG
Detection of Claudin-6 in HEK293 Human Cell Line Transfected with Human Claudin-6 and eGFP by Flow Cytometry.
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Human Claudin-6 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human Claudin-6 transfected cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 342927
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Claudin-6
Met1-Val220
Accession # P56747
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Claudin-6 in HEK293 Human Cell Line Transfected with Human Claudin-6 and eGFP by Flow Cytometry. View Larger

Detection of Claudin-6 in HEK293 Human Cell Line Transfected with Human Claudin-6 and eGFP by Flow Cytometry. HEK293 human cell line transfected with Human Claudin-6 and eGFP was stained with (A) Mouse Anti-Human Claudin-6 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB3656R)  or (B) Mouse IgG2B Isotype Control Antibody (Catalog # IC0041R). View our protocol for Staining Membrane-associated Proteins.

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Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Claudin-6

Claudin-6 is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Human Claudin-6 shares 88% and 86% amino acid sequence identity with mouse and rat Claudin-6, respectively.

Entrez Gene IDs
9074 (Human); 54419 (Mouse); 287098 (Rat)
Alternate Names
claudin 6; Claudin6; Claudin-6; CLDN6; Skullin 2; skullin

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