Human Claudin-6 Alexa Fluor® 647-conjugated Antibody

FAB3656R Datasheet / COA / SDS

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FAB3656R-100UG

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Detection of Claudin-6 in HEK293 Human Cell Line Transfected with Human Claudin-6 and eGFP by Flow Cytometry.

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Human Claudin-6 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Stains human Claudin-6 transfected cells but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2B Clone # 342927

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Claudin-6
Met1-Val220
Accession # P56747

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of Claudin-6 in HEK293 Human Cell Line Transfected with Human Claudin-6 and eGFP by Flow Cytometry. HEK293 human cell line transfected with Human Claudin-6 and eGFP was stained with (A) Mouse Anti-Human Claudin-6 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB3656R) or (B) Mouse IgG2B Isotype Control Antibody (Catalog # IC0041R). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Claudin-6

Claudin-6 is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Human Claudin-6 shares 88% and 86% amino acid sequence identity with mouse and rat Claudin-6, respectively.

Entrez Gene IDs

9074 (Human); 54419 (Mouse); 287098 (Rat)

Alternate Names

claudin 6; Claudin6; Claudin-6; CLDN6; Skullin 2; skullin