Mouse IgG2B Alexa Fluor® 647-conjugated Isotype Control

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R&D Systems Antibodies
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Citations (3)
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Mouse IgG2B Alexa Fluor® 647-conjugated Isotype Control Summary

Selected for use as mouse IgG2B isotype control for flow cytometry applications.
Monoclonal Mouse IgG2B Clone # 133303
Protein A or G purified from hybridoma culture supernatant
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)


Recommended Concentration
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names

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Citations for Mouse IgG2B Alexa Fluor® 647-conjugated Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Irreversible alteration of extracellular vesicle and cell-free messenger RNA profiles in human plasma associated with blood processing and storage
    Authors: HJ Kim, MJ Rames, S Tassi Yung, R Armstrong, M Morita, ATP Ngo, OJT McCarty, F Civitci, TK Morgan, TTM Ngo
    Scientific Reports, 2022-02-08;12(1):2099.
    Species: Human
    Sample Types: Extracellular Vesicles
    Applications: Flow Cytometry
  2. TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions
    Authors: T Leng, HD Akther, CP Hackstein, K Powell, T King, M Friedrich, Z Christofor, S McCuaig, M Neyazi, CV Arancibia-, J Hagel, F Powrie, RS Peres, V Millar, D Ebner, R Lamichhane, J Ussher, TSC Hinks, E Marchi, C Willberg, P Klenerman
    Cell Rep, 2019-09-17;28(12):3077-3091.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Flow Cytometry of Mouse and Human Adipocytes for the Analysis of Browning and Cellular Heterogeneity
    Authors: CE Hagberg, Q Li, M Kutschke, D Bhowmick, E Kiss, IG Shabalina, MJ Harms, O Shilkova, V Kozina, J Nedergaard, J Boucher, A Thorell, KL Spalding
    Cell Rep, 2018-09-04;24(10):2746-2756.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Isotype Control


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Reviews for Mouse IgG2B Alexa Fluor® 647-conjugated Isotype Control

Average Rating: 4.7 (Based on 3 Reviews)

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Mouse IgG2B Alexa Fluor® 647-conjugated Isotype Control
By Anonymous on 08/25/2021
Application: Flow Sample Tested: IPS2 induced pluripotent stem cells Species: Human

Mouse IgG2B Alexa Fluor® 647-conjugated Isotype Control
By Anonymous on 08/10/2021
Application: Flow Sample Tested: limbal stem cell Species: Human

Mouse IgG2B Alexa Fluor® 647-conjugated Isotype Control
By Anonymous on 06/12/2019
Application: Flow Sample Tested: HEK293 human embryonic kidney cell line Species: Human