Human Claudin-6 Antibody

Catalog # Availability Size / Price Qty
MAB3656
MAB3656-SP
Detection of Claudin-6 in HEK293 Human Cell Line Transfected with Human Claudin-6 and eGFP by Flow Cytometry.
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Product Details
Citations (4)
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Human Claudin-6 Antibody Summary

Species Reactivity
Human
Specificity
Stains human Claudin-6 transfected cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 342927
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Claudin-6
Met1-Val220
Accession # P56747
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Claudin-6 antibody in HEK293 Human Cell Line Transfected with Human Claudin-6 and eGFP antibody by Flow Cytometry. View Larger

Detection of Claudin-6 in HEK293 Human Cell Line Transfected with Human Claudin-6 and eGFP by Flow Cytometry. HEK293 human cell line transfected with (A) Human Claudin-6 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human Claudin-6 Monoclonal Antibody (Catalog # MAB3656) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry Claudin-6 antibody in iPS2 Human Induced Pluripotent Stem Cells by Immunocytochemistry (ICC). View Larger

Claudin‑6 in iPS2 Human Induced Pluripotent Stem Cells. Claudin-6 was detected in immersion fixed iPS2 human induced pluripotent stem cells, differentiated into definitive endoderm with StemXVivo Endoderm Kit (Catalog # SC019), using Mouse Anti-Human Claudin-6 Monoclonal Antibody (Catalog # MAB3656) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). SOX17 was also detected using Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF1924). Specific staining of Claudin-6 was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-6

Claudin-6 is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Human Claudin-6 shares 88% and 86% amino acid sequence identity with mouse and rat Claudin-6, respectively.

Entrez Gene IDs
9074 (Human); 54419 (Mouse); 287098 (Rat)
Alternate Names
claudin 6; Claudin6; Claudin-6; CLDN6; Skullin 2; skullin

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Citations for Human Claudin-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Antibody specificity against highly conserved membrane protein Claudin 6 driven by single atomic contact point
    Authors: Brad Screnci, Lewis J. Stafford, Trevor Barnes, Kristen Shema, Samantha Gilman, Rebecca Wright et al.
    iScience
  2. Identification and functional characterization of potential oncofetal targets in human hepatocellular carcinoma
    Authors: Mei-Mei Li, Fan-En Kong, Guang-Meng Li, Yi-Ti He, Xiao-Feng Zhang, Cheng-Yang Zhang et al.
    STAR Protocols
  3. A single mutation in the E2 glycoprotein of hepatitis C virus broadens the claudin specificity for its infection
    Authors: Y Shirasago, H Fukazawa, S Nagase, Y Shimizu, T Mizukami, T Wakita, T Suzuki, H Tani, M Kondoh, T Kuroda, S Yasuda, Y Sato, K Hanada, M Fukasawa
    Scientific Reports, 2022-11-24;12(1):20243.
    Species: Human
    Sample Types:
    Applications: Bioassay
  4. Identification of novel functions for hepatitis C virus envelope glycoprotein E1 in virus entry and assembly
    Authors: J Haddad, Y Rouillé, X Hanoulle, V Descamps, M Hamze, F Dabboussi, TF Baumert, G Duverlie, M Lavie, J Dubuisson
    J. Virol, 2017-03-29;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Functional Assay

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