Human Cystatin F Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB1889G Datasheet / COA / SDS

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FAB1889G-100UG

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All Cystatin F Antibodies

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Human Cystatin F Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cystatin F in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cystatin A, B, C, D, E/M, S, recombinant mouse (rm) Cystatin/Stefin Homolog, rhFetuin A, r

Source

Monoclonal Mouse IgG2b Clone # 292103

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cystatin F
Gly20-His145
Accession # O76096

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Cystatin F

Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a member of the cystatin superfamily (1‑3). Cystatin F is selectively expressed by hematopoietic cells and may be a biomarker for both liver metastasis and inflammatory lung disorders (3, 4). As a cysteine protease inhibitor, it shows selectivity towards cathepsin L and legumain (1, 2, 5). Compared to other secreted cystatins including C, D, E/M, S, SA and SN, which contain two intra disulfide bonds, cystatin F has two extra Cys residues that may be involved in inter disulfide bonds. Indeed, rhCystatin F showed disulfide bond-linked dimer formation, which was also the case for an E. coli expressed fusion protein containing mature human cystatin F and glutathione S-transferase (2).

Entrez Gene IDs

8530 (Human)

Alternate Names

CMAP; CST7; Cystatin 7; cystatin F (leukocystatin); Cystatin F; Cystatin-7; cystatin-F; Cystatin-like metastasis-associated protein; Leukocystatin