Human Cytokeratin 8 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC3165G
Detection of Cytokeratin 8 in HT‑29 Human Cell Line by Flow Cytometry.
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Human Cytokeratin 8 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cytokeratin 8 in flow cytometry.
Source
Monoclonal Mouse IgG1 Clone # LP3K
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
SV-40 transformed human neonatal keratinocyte extract
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Cytokeratin 8 antibody in HT‑29 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Cytokeratin 8 in HT‑29 Human Cell Line by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) (upper panel, negative control) and HT-29 human colon adenocarcinoma cell line (lower panel, positive control) were stained with Mouse Anti-Human Cytokeratin 8 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC3165G, filled histograms) or isotype control antibody (Catalog # IC002G, open histograms). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Cytokeratin 8

Cytokeratin 8 is one of the first cytokeratins expressed in the embryo and exists in adult tissues as a heterodimer with Cytokeratin 18. Cytokeratin 8 is primarily found in the non-squamous epithelia and is expressed in the majority of adenocarcinomas and ductal carcinomas.

Entrez Gene IDs
3856 (Human); 16691 (Mouse); 25626 (Rat)
Alternate Names
CARD2; CK8; CK-8; CYK8; Cytokeratin 8; Cytokeratin-8; EndoA; K2C8; K8; keratin 8; keratin, type II cytoskeletal 8; keratin-8; KO; KRT8; Type-II keratin Kb8

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