Human Cytokeratin 8 Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Cytokeratin 8 in flow cytometry.
  • Source
    Monoclonal Mouse IgG1 Clone # LP3K
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    SV-40 transformed human neonatal keratinocyte extract
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Cytokeratin 8 in HT‑29 Human Cell Line by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) (upper panel, negative control) and HT‑29 human colon adenocarcinoma cell line (lower panel, positive control) were stained with Mouse Anti-Human Cytokeratin 8 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC3165G, filled histograms) or isotype control antibody (Catalog # IC002G, open histograms). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Cytokeratin 8
Cytokeratin 8 is one of the first cytokeratins expressed in the embryo and exists in adult tissues as a heterodimer with Cytokeratin 18. Cytokeratin 8 is primarily found in the non-squamous epithelia and is expressed in the majority of adenocarcinomas and ductal carcinomas.
  • Entrez Gene IDs:
    3856 (Human); 16691 (Mouse); 25626 (Rat)
  • Alternate Names:
    CARD2; CK8; CK-8; CYK8cytokeratin 8; Cytokeratin 8; Cytokeratin-8; EndoA; K2C8; K8cytokeratin-8; keratin 8; keratin, type II cytoskeletal 8; keratin-8; KO; KRT8; Type-II keratin Kb8
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

Ctrl IC002G 3  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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