Human Cytokeratin 8 Antibody

(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Cytokeratin 8.
  • Source
    Monoclonal Mouse IgG1 Clone # LP3K
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    SV-40 transformed human neonatal keratinocyte extract
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot

    Lane, E.B. et al. (1985) Ann. NY Acad. Sci. 455:241. This application was not tested by R&D Systems.

  • Immunohistochemistry
    1-25 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Cytokeratin 8 in MCF‑7 Human Cell Line. Cytokeratin 8 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human Cytokeratin 8 Monoclonal Antibody (Catalog # MAB3165) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
Cytokeratin 8 in Human Colon Cancer Tissue. Cytokeratin 8 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human Cytokeratin 8 Monoclonal Antibody (Catalog # MAB3165) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytokeratin 8
Cytokeratin 8 is one of the first cytokeratins expressed in the embryo and exists in adult tissues as a heterodimer with Cytokeratin 18. Cytokeratin 8 is primarily found in the non-squamous epithelia and is expressed in the majority of adenocarcinomas and ductal carcinomas.
  • Entrez Gene IDs:
    3856 (Human); 16691 (Mouse); 25626 (Rat)
  • Alternate Names:
    CARD2; CK8; CK-8; CYK8cytokeratin 8; Cytokeratin 8; Cytokeratin-8; EndoA; K2C8; K8cytokeratin-8; keratin 8; keratin, type II cytoskeletal 8; keratin-8; KO; KRT8; Type-II keratin Kb8
Related Research Areas
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Average Rating: 3 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunohistochemistry.

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Immunohistochemistry Human Cytokeratin 8 Antibody MAB3165
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 IHC Human Anonymous 12/10/2017
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Immunohistochemistry Human Cytokeratin 8 Antibody MAB3165
Immunohistochemistry: Human Cytokeratin 8 Antibody [MAB3165]

Summary

ApplicationImmunohistochemistry
Sample TestedKidney cancer tissue
SpeciesHuman

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