Human Cytokeratin 8 Antibody

Catalog # Availability Size / Price Qty
MAB3165
MAB3165-SP
Cytokeratin 8  in MCF‑7 Human Cell Line.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human Cytokeratin 8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cytokeratin 8.
Source
Monoclonal Mouse IgG1 Clone # LP3K
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
SV-40 transformed human neonatal keratinocyte extract
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot

Lane, E.B. et al. (1985) Ann. NY Acad. Sci. 455:241. This application was not tested by R&D Systems.

 
Immunohistochemistry
1-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Cytokeratin 8  in MCF‑7 Human Cell Line. View Larger

Cytokeratin 8 in MCF‑7 Human Cell Line. Cytokeratin 8 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human Cytokeratin 8 Monoclonal Antibody (Catalog # MAB3165) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Cytokeratin 8  in Human Colon Cancer Tissue. View Larger

Cytokeratin 8 in Human Colon Cancer Tissue. Cytokeratin 8 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human Cytokeratin 8 Monoclonal Antibody (Catalog # MAB3165) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytokeratin 8

Cytokeratin 8 is one of the first cytokeratins expressed in the embryo and exists in adult tissues as a heterodimer with Cytokeratin 18. Cytokeratin 8 is primarily found in the non-squamous epithelia and is expressed in the majority of adenocarcinomas and ductal carcinomas.

Entrez Gene IDs
3856 (Human); 16691 (Mouse); 25626 (Rat)
Alternate Names
CARD2; CK8; CK-8; CYK8cytokeratin 8; Cytokeratin 8; Cytokeratin-8; EndoA; K2C8; K8cytokeratin-8; keratin 8; keratin, type II cytoskeletal 8; keratin-8; KO; KRT8; Type-II keratin Kb8

Product Datasheets

Citation for Human Cytokeratin 8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. TGF?1 in fibroblasts-derived exosomes promotes epithelial-mesenchymal transition of ovarian cancer cells
    Authors: W Li, X Zhang, J Wang, M Li, C Cao, J Tan, D Ma, Q Gao
    Oncotarget, 2017;8(56):96035-96047.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG1 Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Cytokeratin 8 Antibody

Average Rating: 3 (Based on 1 Review)

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Human Cytokeratin 8 Antibody
By Anonymous on 12/10/2017
Application: IHC Sample Tested: Kidney cancer tissue Species: Human