Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

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Detection of Mouse IgG Isotype Control by Flow Cytometry
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Product Details
Citations (8)
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Mouse IgG1 Alexa Fluor® 488-conjugated Antibody Summary

Selected for use as mouse IgG1 isotype control for flow cytometry applications.
Monoclonal Mouse IgG1 kappa Clone # 11711
Protein A or G purified from hybridoma culture supernatant
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)


Recommended Concentration
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Detection of Mouse IgG Control by Flow Cytometry BG01V human embryonic stem cells were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1448G, filled histogram) or Mouse IgG Alexa Fluor® 488-conjugated Isotype Control Antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G1
Alternate Names
IgG1; Igh-4; RGD1359539; VH7183

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Citations for Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states
    Authors: NM Dräger, SM Sattler, CT Huang, OM Teter, K Leng, SH Hashemi, J Hong, G Aviles, CD Clelland, L Zhan, JC Udeochu, L Kodama, AB Singleton, MA Nalls, J Ichida, ME Ward, F Faghri, L Gan, M Kampmann
    Nature Neuroscience, 2022-08-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Differential Marker Expression between Keratinocyte Stem Cells and Their Progeny Generated from a Single Colony
    Authors: D Ali, D Alhattab, H Jafar, M Alzubide, N Sharar, S Bdour, A Awidi
    International Journal of Molecular Sciences, 2021-10-06;22(19):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. High-throughput single-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection of nucleic acids, proteins, and their combinations
    Authors: J Zhou, Z Wu, J Hu, D Yang, X Chen, Q Wang, J Liu, M Dou, W Peng, Y Wu, W Wang, C Xie, M Wang, Y Song, H Zeng, C Bai
    Sci Adv, 2020-11-20;6(47):.
    Species: Human
    Sample Types: Extracellular Vesicles
    Applications: ICC
  4. Human NK Cells Lyse Th2-Polarizing Dendritic Cells via NKp30 and DNAM-1
    Authors: K Walwyn-Bro, K Guldevall, M Saeed, D Pende, B Önfelt, AS MacDonald, DM Davis
    J. Immunol., 2018-08-17;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Dual role of DR5 in death and survival signaling leads to TRAIL resistance in cancer cells
    Authors: Y Shlyakhtin, V Pavet, H Gronemeyer
    Cell Death Dis, 2017-08-31;8(8):e3025.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Induction and Differentiation of IL-10-Producing Regulatory B Cells from Healthy Blood Donors and Rheumatoid Arthritis Patients.
    Authors: Banko Z, Pozsgay J, Szili D, Toth M, Gati T, Nagy G, Rojkovich B, Sarmay G
    J Immunol, 2017-01-13;198(4):1512-1520.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  7. Induction of indoleamine 2,3-dioxygenase by Borrelia burgdorferi in human immune cells correlates with pathogenic potential.
    Authors: Love A, Schwartz I, Petzke M
    J Leukoc Biol, 2014-11-24;97(2):379-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells.
    Authors: Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, Sakuma T, Ohmine S, Terzic A, Ikeda Y
    Mol Ther, 2012-11-27;21(1):228-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control


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Reviews for Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

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Mouse IgG1 Alexa Fluor® 488-conjugated Antibody
By Anonymous on 02/02/2023
Application: Flow Sample Tested: Human tissue Species: Human

Use as Iso-type Ctrl

Mouse IgG1 Alexa Fluor® 488-conjugated Antibody
By Tahmineh Safaie on 11/08/2019
Application: Flow Sample Tested: MCF-7 human breast cancer cell line Species: Human

MCF, cells were fixed and permeabilized and the stained with the antibody and the intracellular expression was measured by Flow Cytometry

Mouse IgG1 Alexa Fluor® 488-conjugated Antibody
By Tahmineh Safaie on 11/08/2019
Application: Flow Sample Tested: B16-F1 mouse melanoma cell line Species: Mouse

B16F10 cells were stained extracellularly with the antibodies and Expression was measured by Flow cytometry.

Mouse IgG1 Alexa Fluor® 488-conjugated Antibody
By Anonymous on 08/21/2018
Application: Flow Sample Tested: Raji human Burkitt's lymphoma cell line Species: Human