Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC002G
Detection of Mouse IgGControl by Flow Cytometry
1 Image
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Mouse IgG1 Alexa Fluor® 488-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG1 isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG1 kappa Clone # 11711
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Mouse IgGControl by Flow Cytometry BG01V human embryonic stem cells were stained with MouseAnti-Human Alkaline Phosphatase/ALPL Alexa Fluor® 488‑conjugatedMonoclonal Antibody (Catalog # FAB1448G,filled histogram) or Mouse IgG Alexa Fluor® 488-conjugated Isotype Control Antibody(Catalog # IC002G, open histogram). View our protocol for StainingMembrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G1
Entrez Gene IDs
101867415 (Cynomolgus Monkey)
Alternate Names
IgG1

Product Datasheets

Citations for Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Human NK Cells Lyse Th2-Polarizing Dendritic Cells via NKp30 and DNAM-1
    Authors: K Walwyn-Bro, K Guldevall, M Saeed, D Pende, B Önfelt, AS MacDonald, DM Davis
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. Dual role of DR5 in death and survival signaling leads to TRAIL resistance in cancer cells
    Authors: Y Shlyakhtin, V Pavet, H Gronemeyer
    Cell Death Dis, 2017;8(8):e3025.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  3. Induction and Differentiation of IL-10-Producing Regulatory B Cells from Healthy Blood Donors and Rheumatoid Arthritis Patients.
    Authors: Banko Z, Pozsgay J, Szili D, Toth M, Gati T, Nagy G, Rojkovich B, Sarmay G
    J Immunol, 2017;198(4):1512-1520.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Ctrl
  4. Induction of indoleamine 2,3-dioxygenase by Borrelia burgdorferi in human immune cells correlates with pathogenic potential.
    Authors: Love A, Schwartz I, Petzke M
    J Leukoc Biol, 2015;97(2):379-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  5. Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells.
    Authors: Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, Sakuma T, Ohmine S, Terzic A, Ikeda Y
    Mol Ther, 2013;21(1):228-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow control

FAQs

No product specific FAQs exist for this product, however you may

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Reviews for Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

Average Rating: 5 (Based on 1 Review)

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Mouse IgG1 Alexa Fluor® 488-conjugated Antibody
By Anonymous on 08/21/2018
Application: Flow Sample Tested: Raji human Burkitt's lymphoma cell line Species: Human