Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

(3 citations)   
  • Specificity
    Selected for use as mouse IgG1 isotype control for flow cytometry applications.
  • Source
    Monoclonal Mouse IgG1 kappa Clone # 11711
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse IgGControl by Flow Cytometry BG01V human embryonic stem cells were stained with MouseAnti-Human Alkaline Phosphatase/ALPL Alexa Fluor® 488‑conjugatedMonoclonal Antibody (Catalog # FAB1448G,filled histogram) or Mouse IgG Alexa Fluor® 488-conjugated Isotype Control Antibody(Catalog # IC002G, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G1
  • Entrez Gene IDs:
    101867415 (Cynomolgus Monkey)
  • Alternate Names:
    IgG1
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Induction and Differentiation of IL-10-Producing Regulatory B Cells from Healthy Blood Donors and Rheumatoid Arthritis Patients.
    Authors: Banko Z, Pozsgay J, Szili D, Toth M, Gati T, Nagy G, Rojkovich B, Sarmay G
    J Immunol, 2017;198(4):1512-1520.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Ctrl
  2. Induction of indoleamine 2,3-dioxygenase by Borrelia burgdorferi in human immune cells correlates with pathogenic potential.
    Authors: Love A, Schwartz I, Petzke M
    J Leukoc Biol, 2015;97(2):379-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells.
    Authors: Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, Sakuma T, Ohmine S, Terzic A, Ikeda Y
    Mol Ther, 2013;21(1):228-39.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow control
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 8  
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Mouse IgG1 Fluorescein-conjugated Antibody

Ctrl IC002F 14  
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Mouse IgG1 Alexa Fluor® 700-conjugated Antibody

Ctrl IC002N  
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Mouse IgG1 PerCP-conjugated Antibody

Ctrl IC002C  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Mouse IgG1 Biotinylated Antibody

Ctrl IC002B 2
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Mouse IgG1 Alexa Fluor® 647-conjugated Antibody

Ctrl IC002R
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Mouse IgG1 Alexa Fluor® 594-conjugated Antibody

Ctrl IC002T
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Mouse IgG1 Alexa Fluor® 750-conjugated Antibody

Ctrl IC002S
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Mouse IgG1 Alexa Fluor® 405-conjugated Antibody

Ctrl IC002V
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