Human Cytokine A Premixed Magnetic Luminex Performance Assay

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  • Assay Type
    Magnetic bead-based multiplex assay for the Luminex® MAGPIX® platform
  • Format
    96-well microplate and antibody-coated magnetic beads*
  • Analytes Detected
    Please see table below.
  • Performance Validation
    For standard curves, intra-assay and inter-assay precision, recovery, and linearity data for the different analyte-specific beads, please see product datasheets for corresponding individual Bead Sets (below).

Analytes

Select analytes you would like to measure from the list below. Please note that analytes with the same bead region are not compatible in the same assay and when selected will populate a new assay after clicking the "continue" button.

** Initial Starting Dilutions

* Note: This analyte is in more than one assay. Please refer to the Technical Datasheet for information specific to this analyte.

For standard curves, intra-assay and inter-assay precision, recovery, and linearity data for the different analyte-specific beads, please see product datasheets for corresponding individual Bead Sets (below).

 

* Antibody-coated beads supplied in compatible Bead Sets, sold separately (see below).

 

Product Features
  • Simultaneously detect multiple analytes
  • Magnetic format allows for easier wash steps
  • User selects specific analytes of interest
  • Requires small sample size
  • Undergoes similar validation testing as Quantikine® ELISA Kits
  • Precise and Specific

 

Base Kit Contents
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent(s)
  • Streptavidin-PE
  • Wash Buffer Concentrate
  • Mixing Bottles
  • Round-bottom Microplate
  • Foil Plate Sealers

 

Bead Set Contents
  • Antibody-coated magnetic beads
  • Biotinylated detection antibody

 

Information for Compatiable Analytes

For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Bead Sets.

Analyte Catalog
Number
Sensitivity
(pg/mL)
High Standard Value*
(pg/mL)
Microparticle
Region
CXCL5/ENA-78 LUHM254 4.14 6,900 12
FGF basic LUHM233 4.91 5,750 13
G-CSF LUHM214 1.48 4,500 14
GM-CSF LUHM215 1.98 2,850 15
IFN-gamma LUHM285 1.27 3,250 18
IL-1 alpha/IL-1F1 LUHM200 0.36 1,850 19
IL-1 beta/IL-1F2 LUHM201 0.57 2,400 20
IL-1ra/IL-1F3 LUHM280 10.91 5,500 21
IL-2 LUHM202 2.23 2,300 22
IL-4 LUHM204 4.46 3,750 25
IL-5 LUHM205 0.71 1,380 26
IL-6 LUHM206 1.11 4,950 27
CXCL8/IL-8 LUHM208 1.97 2,950 28
IL-10 LUHM217 0.30 3,200 29
IL-17 LUHM317 1.10 2,550 30
CCL2/MCP-1 LUHM279 0.47 2,100 33
CCL3/MIP-1 alpha LUHM270 28.30 16,500 34
CCL4/MIP-1 beta LUHM271 0.72 6,200 35
CCL5/RANTES LUHM278 1.91 2,400 36
TNF-alpha LUHM210 1.50 4,500 37
Tpo LUHM288 9.94 16,600 38
VEGF LUHM293 1.84 2,850 39

*A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

 

Validated Samples and Recommended Starting Dilution

Analyte Cell Culture
Supernate
Serum EDTA Plasma Heparin Plasma
CXCL5/ENA-78 None 1:4 1:4 1:4
FGF basic None 1:4 1:4 N/R
G-CSF None 1:4 1:4 1:4
GM-CSF None 1:4 1:4 1:4
IFN-gamma None 1:4 1:4 1:4
IL-1 alpha/IL-1F1 None 1:4 1:4 1:4
IL-1 beta/IL-1F2 None 1:4 1:4 1:4
IL-1ra/IL-1F3 None 1:4 1:4 1:4
IL-2 None 1:4 1:4 1:4
IL-4 None 1:4 1:4 1:4
IL-5 None 1:4 1:4 1:4
IL-6 None 1:4 1:4 1:4
CXCL8/IL-8 None 1:4 1:4 1:4
IL-10 None 1:4 1:4 1:4
IL-17 None 1:4 1:4 1:4
CCL2/MCP-1 None 1:4 1:4 1:4
CCL3/MIP-1 alpha None 1:4 1:4 1:4
CCL4/MIP-1 beta None 1:4 1:4 1:4
CCL5/RANTES None 1:100 1:100 1:100
TNF-alpha None 1:4 1:4 1:4
Tpo None 1:4 1:4 1:4
VEGF None 1:4 1:4 1:4

N/R=This sample type is not recommended for use in this assay

 

More Information about Magnetic Bead-Based Multiple Kits for the Luminex Platform

Magnetic Luminex High Performance Assay multiplex kits are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex® 100/200™ or Bio-Rad® Bio-Plex®, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded magnetic microparticles. Microparticles, standards and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, streptavidin-phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated antibody, is added to each well. A final wash removes unbound Streptavidin-PE, the microparticles are resuspended in buffer and read using the Luminex MAGPIX Analyzer. A magnet in the analyzer captures and holds the superparamagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

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Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

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    Analyte Notes

    Analytes with overlapping bead regions or other incompatibilities will be placed in their own assay.

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