Human DPPIV/CD26 Quantikine ELISA Kit

R&D Systems | Catalog # DC260

R&D Systems
Discontinued Product
DC260 has been discontinued. An alternative/replacement product is available: DC260B. View all DPPIV/CD26 products.

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (0 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (100 µL), Urine (50 µL)

Sensitivity

0.072 ng/mL

Assay Range

0.31-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
View all DPPIV/CD26 ELISA Kits »

Product Summary for Human DPPIV/CD26 Quantikine ELISA Kit

The Quantikine Human DPPIV/CD26 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human DPPIV in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human DPPIV and antibodies raised against the recombinant factor. Results obtained using natural human DPPIV showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human DPPIV.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Species

Human

Specificity

Natural and recombinant human DPPIV

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 2.16 8.15 14.1 2.22 8.25 14.6
Standard Deviation 0.105 0.280 0.916 0.140 0.695 1.18
CV% 4.9 3.4 6.5 6.3 8.4 8.1

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 2.32 8.78 15.8 2.43 8.53 15.9
Standard Deviation 0.142 0.354 1.15 0.207 0.777 1.30
CV% 6.1 4.0 7.3 8.5 9.1 8.2

Recovery for Human DPPIV/CD26 Quantikine ELISA Kit

The recovery of DPPIV spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 87-113
Urine (n=4) 101 88-113

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of DPPIV were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human DPPIV/CD26 ELISA Linearity

Scientific Data Images for Human DPPIV/CD26 Quantikine ELISA Kit

Human DPPIV/CD26 ELISA Cell Culture Supernate/Saliva/Urine Standard Curve

Human DPPIV/CD26 ELISA Cell Culture Supernate/Saliva/Urine Standard Curve

Human DPPIV/CD26 ELISA Serum/Plasma Standard Curve

Human DPPIV/CD26 ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: DPPIV/CD26

DPPIV/CD26 is a serine exopeptidase that is expressed as a noncovalent homodimer on the surface of epithelial cells, endothelial cells, and activated lymphocytes. It regulates multiple aspects of immune and endocrine function by cleaving Xaa-Pro or Xaa-Ala dipeptides from the N-terminus of a wide variety of chemokines (CCL4 and 5, CXCL6, 9, 10, 11, and 12), growth factors (GM-CSF, IL-3), and peptide hormones (Glucagon, Glucagon-like Peptides 1 and 2, GIP, GHRH, Procalcitonin, Neuropeptide Y, and Substance P). DPPIV interacts in cis with Adenosine Deaminase on T cells, in trans with Caveolin-1 on antigen presenting cells, and it serves as a cell entry coreceptor for HIV and coronavirus. It provides costimulatory proliferation and activation signals to both CD4+ and CD8+ T cells. A soluble form of DPPIV can be proteolytically shed from adipocytes, leading to insulin resistance in adipocytes and skeletal muscle.

Long Name

Dipeptidyl-peptidase IV

Alternate Names

CD26, DPP4

Entrez Gene IDs

1803 (Human); 13482 (Mouse); 397492 (Porcine); 102133935 (Cynomolgus Monkey)

Gene Symbol

DPP4

Additional DPPIV/CD26 Products

Product Documents for Human DPPIV/CD26 Quantikine ELISA Kit

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human DPPIV/CD26 Quantikine ELISA Kit

For research use only

Citations for Human DPPIV/CD26 Quantikine ELISA Kit

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