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Human DPPIV/CD26 Quantikine ELISA Kit

Catalog #: DC260
8 Citations Datasheet / COA / SDS
Newer Version Available: DC260B
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Discontinued Product

DC260 has been discontinued and is replaced by DC260B.

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Citations (8)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human DPPIV/CD26 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates ( uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (100 uL), Urine (50 uL)
Sensitivity
0.072 ng/mL
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human DPPIV
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human DPPIV/CD26 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human DPPIV in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human DPPIV and antibodies raised against the recombinant factor. Results obtained using natural human DPPIV showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human DPPIV.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 2.16 8.15 14.1 2.22 8.25 14.6
Standard Deviation 0.105 0.28 0.916 0.14 0.695 1.18
CV% 4.9 3.4 6.5 6.3 8.4 8.1

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 2.32 8.78 15.8 2.43 8.53 15.9
Standard Deviation 0.142 0.354 1.15 0.207 0.777 1.3
CV% 6.1 4 7.3 8.5 9.1 8.2

Recovery

The recovery of DPPIV spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 87-113
Urine (n=4) 101 88-113

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of DPPIV were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human DPPIV/CD26 ELISA Linearity

Scientific Data

N/A View Larger

N/A View Larger

N/A Human DPPIV/CD26 ELISA Cell Culture Supernate/Saliva/Urine Standard Curve View Larger

Human DPPIV/CD26 ELISA Cell Culture Supernate/Saliva/Urine Standard Curve

N/A Human DPPIV/CD26 ELISA Serum/Plasma Standard Curve View Larger

Human DPPIV/CD26 ELISA Serum/Plasma Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: DPPIV/CD26

DPPIV/CD26 is a serine exopeptidase that is expressed as a noncovalent homodimer on the surface of epithelial cells, endothelial cells, and activated lymphocytes. It regulates multiple aspects of immune and endocrine function by cleaving Xaa-Pro or Xaa-Ala dipeptides from the N-terminus of a wide variety of chemokines (CCL4 and 5, CXCL6, 9, 10, 11, and 12), growth factors (GM-CSF, IL-3), and peptide hormones (Glucagon, Glucagon-like Peptides 1 and 2, GIP, GHRH, Procalcitonin, Neuropeptide Y, and Substance P). DPPIV interacts in cis with Adenosine Deaminase on T cells, in trans with Caveolin-1 on antigen presenting cells, and it serves as a cell entry coreceptor for HIV and coronavirus. It provides costimulatory proliferation and activation signals to both CD4+ and CD8+ T cells. A soluble form of DPPIV can be proteolytically shed from adipocytes, leading to insulin resistance in adipocytes and skeletal muscle.

Long Name:
Dipeptidyl-peptidase IV
Entrez Gene IDs:
1803 (Human); 13482 (Mouse); 397492 (Porcine); 102133935 (Cynomolgus Monkey)
Alternate Names:
ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human DPPIV/CD26 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Low levels of soluble DPP4 among Saudis may have constituted a risk factor for MERS endemicity
    Authors: KR Alkharsah, SA Aljaroodi, JU Rahman, AN Alnafie, R Al Dossary, RY Aljindan, AM Alnimr, J Hussen
    PLoS ONE, 2022-04-12;17(4):e0266603.
    Species: Human
    Sample Types: Plasma
  2. Adipokines and inflammatory markers in elderly subjects with high risk of type 2 diabetes and cardiovascular disease
    Authors: T Saukkonen, SJ Mutt, J Jokelainen, AM Saukkonen, GS Raza, T Karhu, P Härkönen, J Eckel, KH Herzig, U Rajala, S Keinänen-K
    Sci Rep, 2018-08-24;8(1):12816.
    Species: Human
    Sample Types: Plasma
  3. Reduction of soluble dipeptidyl peptidase 4 levels in plasma of patients infected with Middle East respiratory syndrome coronavirus
    Authors: KS Inn, Y Kim, A Aigerim, U Park, ES Hwang, MS Choi, YS Kim, NH Cho
    Virology, 2018-03-26;518(0):324-327.
    Species: Human
    Sample Types: Sputum
  4. Correction of vitamin D deficiency facilitated suppression of IP-10 and DPP IV levels in patients with chronic hepatitis C: A randomised double-blinded, placebo-control trial
    Authors: P Komolmit, K Charoensuk, K Thanapirom, S Suksawatam, P Thaimai, C Chirathawo, Y Poovorawan
    PLoS ONE, 2017-04-04;12(4):e0174608.
    Species: Human
    Sample Types: Serum
  5. Dipeptidyl peptidase-IV in chronic heart failure with reduced ejection fraction
    Authors: P Lourenço, S Silva, F Cunha, J Pereira, A Ribeiro, N Silva, JT Guimarães, JP Araújo, P Bettencour
    Int. J. Cardiol, 2017-03-11;0(0):.
    Species: Human
    Sample Types: Serum
  6. Adiponectin, chemerin, cytokines, and dipeptidyl peptidase 4 are released from human adipose tissue in a depot-dependent manner: an in vitro system including human serum albumin.
    Authors: Svensson H, Oden B, Eden S, Lonn M
    BMC Endocr Disord, 2014-01-22;14(0):7.
    Species: Human
    Sample Types: Tissue Homogenates
  7. Early and late effects of the DPP-4 inhibitor vildagliptin in a rat model of post-myocardial infarction heart failure.
    Authors: Yin M, Sillje HH, Meissner M, van Gilst WH, de Boer RA
    Cardiovasc Diabetol, 2011-09-28;10(0):85.
    Species: Human
    Sample Types: Plasma
  8. Serum level of CD26 predicts time to first treatment in early B-chronic lymphocytic leukemia.
    Authors: Molica S, Digiesi G, Mirabelli R, Cutrona G, Antenucci A, Molica M, Giannarelli D, Sperduti I, Morabito F, Neri A, Baldini L, Ferrarini M
    Eur. J. Haematol., 2009-05-02;83(0):208-14.
    Species: Human
    Sample Types: Serum

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