< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
The Quantikine® Human DPPIV/CD26 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human DPPIV in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human DPPIV, and antibodies raised against the recombinant factor. Results obtained using natural human DPPIV showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human DPPIV.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Saliva, Urine
Serum, EDTA Plasma, Heparin Plasma
The recovery of human DPPIV spiked to levels throughout the range of the assay in various matrices was evaluated.
Average % Recovery
Cell Culture Media (n=4)
To assess linearity of the assay, samples containing and/or spiked with high concentrations of human DPPIV were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Dipeptidyl peptidase IV (DPPIV/CD26) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of proteins and peptides. It plays an important role in T-cell costimulation, chemokine biology, type II diabetes and tumor biology. There are at least 15 DPPIV-related human genes. Some function as enzymes, including DPP8, DPP9, fibroblast activation protein (FAP), prolyl endopeptidase (PREP), and N-acylaminoacyl-peptide hydrolase (APEH) while others (DPP6/DPPX and DPP10/DPRP3) may have lost their protease properties but acquired novel functions. For example, DPP6 is a critical component of neuronal A-type K+ channels.
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