Human DPPIV/CD26 Quantikine ELISA Kit DC260B: R&D Systems
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates ( uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (100 uL), Urine (50 uL)
  • Sensitivity
    0.072 ng/mL
  • Assay Range
    0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
  • Specificity
    Natural and recombinant human DPPIV
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Human DPPIV/CD26 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human DPPIV in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human DPPIV and antibodies raised against the recombinant factor. Results obtained using natural human DPPIV showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human DPPIV.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Saliva, Urine
Intra-Assay Precision Inter-Assay Precision
Sample112233112233
n202020202020404040404040
Mean2.162.168.158.1514.114.12.222.228.258.2514.614.6
Standard Deviation0.1050.1050.280.280.9160.9160.140.140.6950.6951.181.18
CV%4.94.93.43.46.56.56.36.38.48.48.18.1

Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean2.328.7815.82.438.5315.9
Standard Deviation0.1420.3541.150.2070.7771.3
CV%6.147.38.59.18.2

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean2.328.7815.82.438.5315.9
Standard Deviation0.1420.3541.150.2070.7771.3
CV%6.147.38.59.18.2

Recovery

The recovery of DPPIV spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 87-113
Cell Culture Media (n=4) 101 87-113
Cell Culture Media (n=4) 101 87-113
Cell Culture Media (n=4) 101 87-113
Urine (n=4) 101 88-113
Urine (n=4) 101 88-113
Urine (n=4) 101 88-113
Urine (n=4) 101 88-113
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of DPPIV were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human DPPIV/CD26 Quantikine ELISA Kit
Human DPPIV/CD26 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: DPPIV/CD26
Dipeptidyl peptidase IV (DPPIV/CD26) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of proteins and peptides. It plays an important role in T-cell costimulation, chemokine biology, type II diabetes and tumor biology. There are at least 15 DPPIV-related human genes. Some function as enzymes, including DPP8, DPP9, fibroblast activation protein (FAP), prolyl endopeptidase (PREP), and N-acylaminoacyl-peptide hydrolase (APEH) while others (DPP6/DPPX and DPP10/DPRP3) may have lost their protease properties but acquired novel functions. For example, DPP6 is a critical component of neuronal A-type K+ channels.
    • Long Name
      Dipeptidyl-peptidase IV
    • Entrez Gene IDs
      1803 (Human); 13482 (Mouse); 102133935 (Cynomolgus Monkey);
    • Alternate Names
      ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    3 Citations: Showing 1 - 3
    Filter your results:

    Species
    Sample Type
    1. Adiponectin, chemerin, cytokines, and dipeptidyl peptidase 4 are released from human adipose tissue in a depot-dependent manner: an in vitro system including human serum albumin.
      Authors: Svensson H, Oden B, Eden S, Lonn M
      BMC Endocr Disord, 2014;14(0):7.
      Species: Human
      Sample Type: Tissue Homogenates
    2. Early and late effects of the DPP-4 inhibitor vildagliptin in a rat model of post-myocardial infarction heart failure.
      Authors: Yin M, Sillje HH, Meissner M, van Gilst WH, de Boer RA
      Cardiovasc Diabetol, 2011;10(0):85.
      Species: Human
      Sample Type: Plasma
    3. Serum level of CD26 predicts time to first treatment in early B-chronic lymphocytic leukemia.
      Authors: Molica S, Digiesi G, Mirabelli R, Cutrona G, Antenucci A, Molica M, Giannarelli D, Sperduti I, Morabito F, Neri A, Baldini L, Ferrarini M
      Eur. J. Haematol., 2009;83(0):208-14.
      Species: Human
      Sample Type: Serum
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