Human DPPIV/CD26 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DC260B
Human DPPIV/CD26 Quantikine ELISA Kit Data
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Citations (7)
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Human DPPIV/CD26 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (50 uL)
Sensitivity
0.072 ng/mL
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human DPPIV.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human DPPIV/CD26 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human DPPIV in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human DPPIV, and antibodies raised against the recombinant factor. Results obtained using natural human DPPIV showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human DPPIV.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 2.16 8.15 14.1 2.22 8.25 14.6
Standard Deviation 0.105 0.28 0.916 0.14 0.695 1.18
CV% 4.9 3.4 6.5 6.3 8.4 8.1

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 2.32 8.78 15.8 2.43 8.53 15.9
Standard Deviation 0.142 0.354 1.15 0.207 0.777 1.3
CV% 6.1 4 7.3 8.5 9.1 8.2

Recovery

The recovery of human DPPIV spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 87-113
Urine (n=4) 101 88-113

Linearity

To assess linearity of the assay, samples containing and/or spiked with high concentrations of human DPPIV were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human DPPIV/CD26 ELISA Linearity

Data Examples

Human DPPIV/CD26 ELISA Standard Curve

Human DPPIV/CD26 ELISA Standard Curve

Human DPPIV/CD26 ELISA Cell Culture Supernate/Saliva/Urine Standard Curve

Human DPPIV/CD26 ELISA Serum/Plasma Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DPPIV/CD26

DPPIV/CD26 is a serine exopeptidase that is expressed as a noncovalent homodimer on the surface of epithelial cells, endothelial cells, and activated lymphocytes. It regulates multiple aspects of immune and endocrine function by cleaving Xaa-Pro or Xaa-Ala dipeptides from the N-terminus of a wide variety of chemokines (CCL4 and 5, CXCL6, 9, 10, 11, and 12), growth factors (GM-CSF, IL-3), and peptide hormones (Glucagon, Glucagon-like Peptides 1 and 2, GIP, GHRH, Procalcitonin, Neuropeptide Y, and Substance P). DPPIV interacts in cis with Adenosine Deaminase on T cells, in trans with Caveolin-1 on antigen presenting cells, and it serves as a cell entry coreceptor for HIV and coronavirus. It provides costimulatory proliferation and activation signals to both CD4+ and CD8+ T cells. A soluble form of DPPIV can be proteolytically shed from adipocytes, leading to insulin resistance in adipocytes and skeletal muscle.

Long Name:
Dipeptidyl-peptidase IV
Entrez Gene IDs:
1803 (Human); 13482 (Mouse); 102133935 (Cynomolgus Monkey)
Alternate Names:
ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human DPPIV/CD26 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Reduction of soluble dipeptidyl peptidase 4 levels in plasma of patients infected with Middle East respiratory syndrome coronavirus
    Authors: KS Inn, Y Kim, A Aigerim, U Park, ES Hwang, MS Choi, YS Kim, NH Cho
    Virology, 2018;518(0):324-327.
    Species: Human
    Sample Types: Sputum
  2. Adipokines and inflammatory markers in elderly subjects with high risk of type 2 diabetes and cardiovascular disease
    Authors: T Saukkonen, SJ Mutt, J Jokelainen, AM Saukkonen, GS Raza, T Karhu, P Härkönen, J Eckel, KH Herzig, U Rajala, S Keinänen-K
    Sci Rep, 2018;8(1):12816.
    Species: Human
    Sample Types: Plasma
  3. Correction of vitamin D deficiency facilitated suppression of IP-10 and DPP IV levels in patients with chronic hepatitis C: A randomised double-blinded, placebo-control trial
    Authors: P Komolmit, K Charoensuk, K Thanapirom, S Suksawatam, P Thaimai, C Chirathawo, Y Poovorawan
    PLoS ONE, 2017;12(4):e0174608.
    Species: Human
    Sample Types: Serum
  4. Dipeptidyl peptidase-IV in chronic heart failure with reduced ejection fraction
    Authors: P Lourenço, S Silva, F Cunha, J Pereira, A Ribeiro, N Silva, JT Guimarães, JP Araújo, P Bettencour
    Int. J. Cardiol, 2017;0(0):.
    Species: Human
    Sample Types: Serum
  5. Adiponectin, chemerin, cytokines, and dipeptidyl peptidase 4 are released from human adipose tissue in a depot-dependent manner: an in vitro system including human serum albumin.
    Authors: Svensson H, Oden B, Eden S, Lonn M
    BMC Endocr Disord, 2014;14(0):7.
    Species: Human
    Sample Types: Tissue Homogenates
  6. Early and late effects of the DPP-4 inhibitor vildagliptin in a rat model of post-myocardial infarction heart failure.
    Authors: Yin M, Sillje HH, Meissner M, van Gilst WH, de Boer RA
    Cardiovasc Diabetol, 2011;10(0):85.
    Species: Human
    Sample Types: Plasma
  7. Serum level of CD26 predicts time to first treatment in early B-chronic lymphocytic leukemia.
    Authors: Molica S, Digiesi G, Mirabelli R, Cutrona G, Antenucci A, Molica M, Giannarelli D, Sperduti I, Morabito F, Neri A, Baldini L, Ferrarini M
    Eur. J. Haematol., 2009;83(0):208-14.
    Species: Human
    Sample Types: Serum

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