Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 96201
View all Tyro3/Dtk Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dtk
Ala41-Ser428 (predicted)
Accession # Q06418

Specificity

Detects human Dtk in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity or interference with recombinant mouse Dtk, recombinant human (rh) Axl, or rhMer is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Tyro3/Dtk PE‑conjugated Antibody

Detection of Dtk antibody in K562 Human Cell Line antibody by Flow Cytometry.

Detection of Dtk in K562 Human Cell Line by Flow Cytometry.

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Dtk PE-conjugated Monoclonal Antibody (Catalog # FAB859P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Tyro3/Dtk PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: K562 human chronic myelogenous leukemia cell line

Reviewed Applications

Read 2 reviews rated 5 using FAB859P in the following applications:

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Tyro3/Dtk

Dtk (also known as Sky, Tyro3, Rse, Brt), Axl (also known as Ufo, Ark) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor Protein S. The binding affinities for Gas6 are in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

References

  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Long Name

Tyrosine Protein Kinase Receptor TYRO3

Alternate Names

Brt, Dtk, Rse, Sky

Entrez Gene IDs

7301 (Human); 22174 (Mouse); 102135978 (Cynomolgus Monkey)

Gene Symbol

TYRO3

UniProt

Q06418

Additional Tyro3/Dtk Products

Product Documents for Human Tyro3/Dtk PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human Tyro3/Dtk PE‑conjugated Antibody

For research use only

Citations for Human Tyro3/Dtk PE‑conjugated Antibody

Customer Reviews for Human Tyro3/Dtk PE‑conjugated Antibody (2)

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  • Human Tyro3/Dtk PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 06/24/2018
    the antibody was used to stain potential zika virus receptor Tyro3/Dtk in PMID: 29262327. it works very well.
  • Human Dtk PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: iDC human immature myeloid dendritic cells
    Species: Human
    Verified Customer | Posted 09/10/2016
    Human Tyro3/Dtk PE‑conjugated Antibody FAB859P

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