Human Tyro3/Dtk Antibody

Catalog # Availability Size / Price Qty
MAB859-SP
MAB859-500
MAB859-100

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Detection of Dtk in K562 Human Cell Line by Flow Cytometry.
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Product Details
Citations (8)
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Human Tyro3/Dtk Antibody Summary

Species Reactivity
Human
Specificity
Detects human Tyro3/Dtk in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity or interferance with recombinant mouse Tyro3/Dtk, recombinant human (rh) Axl, or rhMer is observed.
Source
Monoclonal Mouse IgG1 Clone # 96201
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tyro3/Dtk
Ala41-Ser428 (predicted)
Accession # Q06418
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human kidney
Knockout Validated
Tyro3/Dtk is specifically detected in K562 myelogenous leukemia parental cell line but is not detectable in Tyro3/Dtk knockout K562 cell line.
 

Human Tyro3/Dtk Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Tyro3/Dtk Biotinylated Antibody (Catalog # BAF859)

Standard: Recombinant Human Tyro3/Dtk Fc Chimera Protein, CF (Catalog # 859-DK)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Dtk antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Dtk in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Dtk Monoclonal Antibody (Catalog # MAB859, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Knockout Validated Tyro3/Dtk Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line. View Larger

Tyro3/Dtk Specificity is Shown by Flow Cytometry in Knockout Cell Line. Tyro3/Dtk knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human Tyro3/Dtk Monoclonal Antibody (Catalog # MAB859, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the Tyro3/Dtk knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tyro3/Dtk

Dtk (also called Sky, Tyro3, Rse, Brt), Axl (Ufo, Ark) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

References
  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Tyrosine Protein Kinase Receptor TYRO3
Entrez Gene IDs
7301 (Human); 22174 (Mouse)
Alternate Names
Brt; BYK; Dtk; EC 2.7.10; EC 2.7.10.1; FLJ16467; Rse; Sky; Tif; TYRO3 protein tyrosine kinase; Tyro3; Tyrosine-protein kinase byk; Tyrosine-protein kinase DTK; tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY

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Citations for Human Tyro3/Dtk Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. CD14+CD16- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK
    Authors: J Xue, L Xu, H Zhu, M Bai, X Li, Z Zhao, H Zhong, G Cheng, X Li, F Hu, Y Su
    Arthritis Research & Therapy, 2020;22(1):221.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication
    Authors: KM Aagaard, A Lahon, MA Suter, RP Arya, MD Seferovic, MB Vogt, M Hu, F Stossi, MA Mancini, RA Harris, M Kahr, C Eppes, M Rac, MA Belfort, CS Park, D Lacorazza, R Rico-Hesse
    Sci Rep, 2017;7(0):41389.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation
    Authors: SN Cross, JA Potter, P Aldo, JY Kwon, M Pitruzzell, M Tong, S Guller, CV Rothlin, G Mor, VM Abrahams
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry.
    Authors: Shimojima M, Stroher U, Ebihara H, Feldmann H, Kawaoka Y
    J. Virol., 2012;86(4):2067-78.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
    J. Exp. Med., 2012;209(11):2033-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques.
    Authors: Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D
    Thromb. Haemost., 2011;107(1):140-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor.
    Authors: Zhu D, Wang Y, Singh I
    Blood, 2010;115(23):4963-72.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Up-regulation of soluble Axl and Mer receptor tyrosine kinases negatively correlates with Gas6 in established multiple sclerosis lesions.
    Authors: Weinger JG, Omari KM, Marsden K, Raine CS, Shafit-Zagardo B
    Am. J. Pathol., 2009;175(1):283-93.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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