Frizzled-10, also known as CD350, is a 68 kDa seven pass transmembrane glycoprotein in the Frizzled family of Wnt receptors (1, 2). The 205 amino acid N-terminal extracellular region of Frizzled-10 contains a cysteine-rich domain that comprises the Wnt binding domain and mediates receptor oligomerization (3‑5). The C-terminal cytoplasmic tail contains a PDZ-interaction motif (3). PDZ motifs mediate intracellular binding to scaffolding proteins. Within the cysteine-rich domain, human Frizzled‑10 shares 71% amino acid (aa) sequence identity with Frizzled-9 and 31%-46% with Frizzled-1, -2, -3, -4, -5, - 6, -7, and -8. It shares 96%, 94%, 90%, and 82% aa sequence identity with chick, mouse, Xenopus, and zebrafish Frizzled-10, respectively. Frizzled-10 is expressed during embryogenesis in the primitive streak, dorsal neural tube, developing brain, limb bud, and airway epithelium (6-11). It is induced by Shh and colocalizes with Shh and Wnt-7a in the neural tube (12, 13). In the adult, Frizzled-10 is expressed in placenta, gastric glands, and colon and renal tubule epithelial cells (4). Frizzled-10 associates with LRP5 to transduce Wnt-7a and Wnt-7b signals, resulting in the stabilization of cytoplasmic beta-catenin (11, 13). Frizzled-10 is also up-regulated in some cancers and transformed cell lines (4, 14). It binds hypoxia inducible gene 2, which promotes oncogenic Wnt signaling and functions as an autocrine growth factor for renal cell carcinomas (15).
Human Frizzled‑10 Alexa Fluor® 647‑conjugated Antibody
R&D Systems | Catalog # FAB34581R
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ile21-Gly161
Accession # Q6NSL8
Specificity
Clonality
Host
Isotype
Applications for Human Frizzled‑10 Alexa Fluor® 647‑conjugated Antibody
Flow Cytometry
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Frizzled-10
References
- Kikuchi, A. et al. (2007) Cell. Signal. 19:659.
- Wang, H. et al. (2006) Cell. Signal. 18:934.
- Koike, J. et al. (1999) Biochem. Biophys. Res. Commun. 262:39.
- Nagayama, S. et al. (2005) Oncogene 24:6201.
- Dann, C.E. et al. (2001) Nature 412:86.
- Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
- Kawakami, Y. et al. (2000) Mech. Dev. 91:375.
- Nasevicius, A. et al. (2000) Mech. Dev. 92:311.
- Moriwaki, J. et al. (2000) Biochem. Biophys. Res. Commun. 278:377.
- Stark, M.R. et al. (2000) Mech. Dev. 93:195.
- Wang, Z. et al. (2005) Mol. Cell. Biol. 25:5022.
- Nunnally, A.P. and B.A. Parr (2004) Dev. Genes Evol. 214:144.
- Kawakami, Y. et al. (2000) Dev. Growth Differ. 42:561.
- Saitoh, T. et al. (2002) Int. J. Oncol. 20:117.
- Togashi, A. et al. (2005) Cancer Res. 65:4817.
Alternate Names
Gene Symbol
UniProt
Additional Frizzled-10 Products
Product Documents for Human Frizzled‑10 Alexa Fluor® 647‑conjugated Antibody
Certificate of Analysis
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Product Specific Notices for Human Frizzled‑10 Alexa Fluor® 647‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
Related Research Areas
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