DLGAP1 (Disks Large-associated protein 1; also SAPAP1, GKAP and SAP90 binding protein) is an intracellular, 117-130 kDa member of the SAPAP family of proteins. It is widely expressed (astrocytes, neurons, fibroblasts and epithelium), and serves as a platform for dynein-DGL1 interaction. This is important during cell migration where the centrosome must be positioned between the nucleus and direction of migration in response to microtubule formation under the protruding edge of the cell membrane. DLGAP1 first binds DLG-1 and then recruits dynein, with a resultant interaction that allows for microtubule anchoring and centrosome positioning. Human DLGAP1 is 977 amino acids (aa) in length. It contains multiple 14 aa acidic repeats, three Pro-rich segments, and at least 14 potential phosphorylation sites. Multiple potential splice variants are reported. In various combinations, there may be a 17, 27 and 31 aa substitution for aa 1-319, a deletion of either aa 531-540 or 531-558, a 24 aa substitution for aa 1-318, and a 13 aa substitution for aa 531-977. Most appear to generate 70-80 kDa isoforms in SDS-PAGE. Over aa 368-472, human and mouse DLGAP1 are identical in aa sequence.
Human GKAP/DLGAP1 Alexa Fluor® 405‑conjugated Antibody
R&D Systems | Catalog # AF7296V
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Lys368-Glu472
Accession # O14490
Specificity
Clonality
Host
Isotype
Applications for Human GKAP/DLGAP1 Alexa Fluor® 405‑conjugated Antibody
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: GKAP/DLGAP1
Long Name
Alternate Names
Gene Symbol
UniProt
Additional GKAP/DLGAP1 Products
Product Documents for Human GKAP/DLGAP1 Alexa Fluor® 405‑conjugated Antibody
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Product Specific Notices for Human GKAP/DLGAP1 Alexa Fluor® 405‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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Protocols
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