Human GKAP/DLGAP1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human GKAP/DLGAP1 by Western Blot.
1 Image
Product Details
Supplemental Products

Human GKAP/DLGAP1 Antibody Summary

Species Reactivity
Detects human GKAP/DLGAP1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human DLGAP2 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human GKAP/DLGAP1
Accession # O14490
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human GKAP/DLGAP1 antibody by Western Blot. View Larger

Detection of Human GKAP/DLGAP1 by Western Blot. Western blot shows lysates of human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GKAP/DLGAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7296) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific band were detected for GKAP/DLGAP1 at approximately 80 kDa and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GKAP/DLGAP1

DLGAP1 (Disks Large-associated protein 1; also SAPAP1, GKAP and SAP90 binding protein) is an intracellular, 117-130 kDa member of the SAPAP family of proteins. It is widely expressed (astrocytes, neurons, fibroblasts and epithelium), and serves as a platform for dynein-DGL1 interaction. This is important during cell migration where the centrosome must be positioned between the nucleus and direction of migration in response to microtubule formation under the protruding edge of the cell membrane. DLGAP1 first binds DLG-1 and then recruits dynein, with a resultant interaction that allows for microtubule anchoring and centrosome positioning. Human DLGAP1 is 977 amino acids (aa) in length. It contains multiple 14 aa acidic repeats, three Pro-rich segments, and at least 14 potential phosphorylation sites. Multiple potential splice variants are reported. In various combinations, there may be a 17, 27 and 31 aa substitution for aa 1-319, a deletion of either aa 531-540 or 531-558, a 24 aa substitution for aa 1-318, and a 13 aa substitution for aa 531-977. Most appear to generate 70-80 kDa isoforms in SDS-PAGE. Over aa 368-472, human and mouse DLGAP1 are identical in aa sequence.

Long Name
Guanylate Kinase-associated Protein
Entrez Gene IDs
9229 (Human); 224997 (Mouse); 65040 (Rat)
Alternate Names
DAP1; DAP-1-ALPHA; DAP-1DAP-1-BETA; discs, large (Drosophila) homolog-associated protein 1; disks large-associated protein 1; DLGAP1; GKAP; GKAPMGC88156; hGKAP; PSD-95/SAP90 binding protein 1; PSD-95/SAP90-binding protein 1; SAP90/PSD-95-associated protein 1; SAPAP1; SAPAP1Guanylate kinase-associated protein

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human GKAP/DLGAP1 Antibody

There are currently no reviews for this product. Be the first to review Human GKAP/DLGAP1 Antibody and earn rewards!

Have you used Human GKAP/DLGAP1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review